Novel Escherichia coli hybrids with enhanced butanol tolerance

被引：23

作者：

Winkler, James ^{[1
]}

Rehmann, Matthew

Kao, Katy C. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem Engn, College Stn, TX 77843 USA

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 07期

基金：

美国国家科学基金会;

关键词：

Butanol tolerance; E; coli; Intergeneric hybrids; L; brevis; Protoplast fusion; INTERGENERIC PROTOPLAST FUSION; LACTIC-ACID BACTERIA; CLOSTRIDIUM-ACETOBUTYLICUM; LACTOBACILLUS; STRAINS;

D O I：

10.1007/s10529-010-0247-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hybrids between Escherichia coli and Lactobacillus brevis were generated via protoplast fusion. Growth kinetics of five hybrid strains and E. coli were used to evaluate the butanol tolerance of the novel strains under different conditions. The hybrid strains tolerated up to 2% (v/v) butanol compared to the 1% (v/v) maximum for E. coli. The growth inhibitory effects of butanol were also significantly less in several of the hybrids compared to E. coli. These results demonstrate the potential use of protoplast fusion to generate butanol-tolerant strains.

引用

页码：915 / 920

页数：6

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