Use of coherent control methods through scattering biological tissue to achieve functional imaging

被引：81

作者：

Dela Cruz, JM

Pastirk, I

Comstock, M

Lozovoy, VV

Dantus, M ^{[1
]}

机构：

[1] Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA

[2] Michigan State Univ, Dept Phys & Astron, E Lansing, MI 48824 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2004年 / 101卷 / 49期

关键词：

pulse shaping; two-photon; femtosecond laser; intrapulse interference;

D O I：

10.1073/pnas.0407733101

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We test whether coherent control methods based on ultrashortpulse phase shaping can be applied when the laser light propagates through biological tissue. Our results demonstrate experimentally that the spectral-phase properties of shaped laser pulses optimized to achieve selective two-photon excitation survive as the laser pulses propagate through tissue. This observation is used to obtain functional images based on selective two- photon excitation of a pH-sensitive chromophore in a sample that is placed behind a slice of biological tissue. Our observation of coherent control through scattering tissue suggests possibilities in multiphoton-based imaging and photodynamic therapy.

引用

页码：16996 / 17001

页数：6

共 34 条

[1] Control of chemical reactions by feedback-optimized phase-shaped femtosecond laser pulses [J].