Mutilation of RNA phage Qβ virus-like particles:: from icosahedrons to rods

被引:60
作者
Cielens, I [1 ]
Ose, V [1 ]
Petrovskis, I [1 ]
Strelnikova, A [1 ]
Renhofa, R [1 ]
Kozlovska, T [1 ]
Pumpens, P [1 ]
机构
[1] Univ Latvia, Biomed Res & Study Ctr, LV-1067 Riga, Latvia
关键词
RNA phage Q beta; virus-like particle; self-assembly; icosahedron; rod-like structure;
D O I
10.1016/S0014-5793(00)02061-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Icosahedral virus-like particles (VLPs) of RNA phage Q beta are stabilized by four disulfide bonds of cysteine residues 74 and 80 within the loop between beta-strands F and G (FG loop) of the monomeric subunits, which determine the five-fold and quasi-six-fold symmetry contacts of the VLPs. In order to reduce the stability of Q beta VLPs, we mutationally converted the amino acid stretch 76-ANGSCD-81 within the FG loop into the 76-VGGVEL-81 sequence. It led to production in Escherichia coli cells of aberrant rod-like Q beta VLPs, along with normal icosahedral capsids. The length of the rod-like particles exceeded 4-30 times the diameter of icosahedral Q beta VLPs. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:261 / 264
页数:4
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