Rosiglitazone upregulates caveolin-1 expression in THP-1 cells through a PPAR-dependent mechanism

被引:58
作者
Llaverias, G
Vázquez-Carrera, M
Sánchez, RM
Noé, W
Ciudad, CJ
Laguna, JC
Alegret, M [1 ]
机构
[1] Univ Barcelona, Fac Farm, Unitat Farmacol, E-08007 Barcelona, Spain
[2] Univ Barcelona, Fac Farm, Dept Bioquim & Biol Mol, E-08007 Barcelona, Spain
关键词
peroxisome proliferator-activated receptors; thiazolidinediones; macrophage; human; liver X receptor;
D O I
10.1194/jlr.M400049-JLR200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Peroxisome proliferator-activated receptor gamma (PPARgamma) activation or overexpression induces caveolin-1 (cav-1) expression in several cell types. The objective of this study was to investigate if PPAR agonists could also regulate the cav-1 gene in macrophages and to explore the mechanisms involved. Our experiments demonstrated that rosiglitazone dose- and time-dependently increased cav-1 mRNA and protein in THP-1 macrophages. This induction was not observed in the presence of inhibitors of transcription or de novo protein synthesis. We also showed that the increase in cav-1 elicited by rosiglitazone was not related either to macrophage differentiation or to cellular apoptosis. The inductive effect seems to be dependent on PPAR activation, as the PPAR antagonist GW9662 abolished it. The activation of the liver X receptor with 22(R)-hydroxycholesterol also increased cav-1 mRNA, whereas the inactive (S) isomer did not.jlr Finally, we identified a functional peroxisome proliferator response element in the cav-1 promoter that was activated upon rosiglitazone treatment in THP-1 macrophages.
引用
收藏
页码:2015 / 2024
页数:10
相关论文
共 42 条
[1]  
Arakawa R, 2000, J LIPID RES, V41, P1952
[2]   Pleiotropic actions of peroxisome proliferator-activated receptors in lipid metabolism and atherosclerosis [J].
Barbier, O ;
Torra, IP ;
Duguay, Y ;
Blanquart, C ;
Fruchart, JC ;
Glineur, C ;
Staels, B .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2002, 22 (05) :717-726
[3]   Direct thiazolidinedione action on isolated rat skeletal muscle fuel handling is independent of peroxisome proliferator-activated receptor-γ-mediated changes in gene expression [J].
Brunmair, B ;
Gras, F ;
Neschen, S ;
Roden, M ;
Wagner, L ;
Waldhäusl, W ;
Fürnsinn, C .
DIABETES, 2001, 50 (10) :2309-2315
[4]   Peroxisome proliferator-activated receptor-γ upregulates caveolin-1 and caveolin-2 expression in human carcinoma cells [J].
Burgermeister, E ;
Tencer, L ;
Liscovitch, M .
ONCOGENE, 2003, 22 (25) :3888-3900
[5]   Nuclear receptors and lipid physiology: Opening the X-files [J].
Chawla, A ;
Repa, JJ ;
Evans, RM ;
Mangelsdorf, DJ .
SCIENCE, 2001, 294 (5548) :1866-1870
[6]   A PPARγ-LXR-ABCA1 pathway in macrophages is involved in cholesterol efflux and atherogenesis [J].
Chawla, A ;
Boisvert, WA ;
Lee, CH ;
Laffitte, BA ;
Barak, Y ;
Joseph, SB ;
Liao, D ;
Nagy, L ;
Edwards, PA ;
Curtiss, LK ;
Evans, RM ;
Tontonoz, P .
MOLECULAR CELL, 2001, 7 (01) :161-171
[7]   PPAR-α and PPAR-γ activators induce cholesterol removal from human macrophage foam cells through stimulation of the ABCA1 pathway [J].
Chinetti, G ;
Lestavel, S ;
Bocher, V ;
Remaley, AT ;
Neve, B ;
Torra, IP ;
Teissier, E ;
Minnich, A ;
Jaye, M ;
Duverger, N ;
Brewer, HB ;
Fruchart, JC ;
Clavey, V ;
Staels, B .
NATURE MEDICINE, 2001, 7 (01) :53-58
[8]   Peroxisome proliferator-activated receptors (PPARs): Nuclear receptors at the crossroads between lipid metabolism and inflammation [J].
Chinetti, G ;
Fruchart, JC ;
Staels, B .
INFLAMMATION RESEARCH, 2000, 49 (10) :497-505
[9]   ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].
DIGNAM, JD ;
LEBOVITZ, RM ;
ROEDER, RG .
NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489
[10]   Peroxisome proliferator-activated receptor-γ:: from adipogenesis to carcinogenesis [J].
Fajas, L ;
Debril, MB ;
Auwerx, J .
JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2001, 27 (01) :1-9