Rapid screening of Aspergillus terreus mutants for overproduction of lovastatin

被引：24

作者：

Ferrón, MAV

López, JLC

Pérez, JAS

Sevilla, JMF

Chisti, Y

机构：

[1] Univ Almeria, Dept Chem Engn, Almeria 04120, Spain

[2] Massey Univ, Inst Technol & Engn, Palmerston North, New Zealand

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2005年 / 21卷 / 02期

关键词：

Aspergillus terreus; Candida albicans; lovastatin; mutation; screening;

D O I：

10.1007/s11274-004-3045-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel rapid screening method is demonstrated for isolating lovastatin-overproducing strains of Aspergillus terreus. The screening methodology, based on the activity of lovastatin against the yeast Candida albicans, is nearly three times as fast as the selection methods used earlier. The new 6-h assay shows a linear correlation between the quantity of lovastatin generated by A. terreus isolates and the inhibition zones obtained on plates of C. albicans. The new technique is less expensive and requires less labour.

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收藏

页码：123 / 125

页数：3

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