Morphine analgesia in the formalin test: reversal by microinjection of quaternary naloxone into the posterior hypothalamic area or periaqueductal gray

被引:53
作者
Manning, BH
Franklin, KBJ
机构
[1] McGill Univ, Dept Psychol, Montreal, PQ H3A 1B1, Canada
[2] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA
基金
加拿大自然科学与工程研究理事会;
关键词
pain; analgesia; morphine; naloxone methobromide; formalin test; posterior hypothalamus; periaqueductal gray;
D O I
10.1016/S0166-4328(97)00130-7
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
Bilateral microinjection of 5 nmol morphine into the posterior hypothalamic area (PHA), periaqueductal gray matter (PAG) or ventral tegmental area (VTA) elicits powerful suppression of nociceptive behaviors in the formalin test, an animal model of injury produced pain. The object of the present study was to determine whether analgesia in the formalin test (50 mu l 2.5% formalin injected s.c. in one hindpaw) induced by systemically administered morphine requires opioid action at these sites, or other putative sites of opioid action. Morphine sulphate (6 mg/kg s.c.) produced almost complete analgesia in the second phase of the formalin test (30-50 min after formalin). Bilateral microinjection of the quaternary opioid antagonist naloxone methobromide (NxBr, 28 ng in 0.5 mu l, 22 min after morphine) into the PHA completely abolished morphine analgesia, while NxBr into FAG partially reversed analgesia. Microinjection of NxBr into the VTA, central nucleus of the amygdala, habenula, striatum, nucleus accumbens or hypothalamic sites outside the PHA did not antagonize morphine analgesia, although microinjections into some of these sites appeared to reduce the cataleptogenic effects of morphine. The data indicate that the PHA and FAG are probably the primary sites of action of morphine in the formalin test. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:97 / 102
页数:6
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