Interactions of putative heparin-binding domains of basic fibroblast growth factor and its receptor, FGFR-1, with heparin using synthetic peptides

被引：28

作者：

Kinsella, L ^{[1
]}

Chen, HL ^{[1
]}

Smith, JA ^{[1
]}

Rudland, PS ^{[1
]}

Fernig, DG ^{[1
]}

机构：

[1] Sch Biol Sci, Canc & Polio Res Fund Labs, Liverpool L69 7ZB, Merseyside, England

来源：

GLYCOCONJUGATE JOURNAL | 1998年 / 15卷 / 04期

关键词：

fibroblast growth factor; FGFR-1; heparin; synthetic peptides;

D O I：

10.1023/A:1006986104865

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined structure-function relationships that have been proposed to account for the heparin-binding properties of basic fibroblast growth factor and its receptor, FGFR-1, using synthetic peptides, DNA synthesis assays and binding assays in a resonant mirror biosensor. The results suggest that the interaction of FGFR-1 with heparin may not be physiologically relevant and that the site of interaction of the polysaccharide on bFGF is more complex than has been anticipated.

引用

页码：419 / 422

页数：4

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