Hydrogen exchange mass spectrometry: what is it and what can it tell us?

被引：149

作者：

Marcsisin, Sean R. ^{[1
,2
]}

Engen, John R. ^{[1
,2
]}

机构：

[1] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA

[2] Northeastern Univ, Barnett Inst Chem & Biol Anal, Boston, MA 02115 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 397卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Deuterium; Protein mass spectrometry; Protein dynamics; Protein conformation; H/D EXCHANGE; DEUTERIUM EXCHANGE; HIV-1; NEF; PROTEINS; DYNAMICS; RESOLUTION; IDENTIFICATION; COVERAGE;

D O I：

10.1007/s00216-010-3556-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins are undoubtedly some of the most essential molecules of life. While much is known about many proteins, some aspects still remain mysterious. One particularly important aspect of understanding proteins is determining how structure helps dictate function. Continued development and implementation of biophysical techniques that provide information about protein conformation and dynamics is essential. In this review, we discuss hydrogen exchange mass spectrometry and how this method can be used to learn about protein conformation and dynamics. The basic concepts of the method are described, the workflow illustrated, and a few examples of its application are provided.

引用

页码：967 / 972

页数：6

共 31 条

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