Inhibition of PCR by agar from bacteriological transport media

被引:35
作者
Gibb, AP
Wong, S
机构
[1] Foothills Hosp, Dept Microbiol & Infect Dis, Calgary, AB T2N 2T9, Canada
[2] Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
[3] Prov Lab Publ Hlth So Alberta, Calgary, AB, Canada
关键词
D O I
10.1128/JCM.36.1.275-276.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We observed inhibition of PCR in throat swabs submitted in routine bacteriological transport media. Experimental studies showed that agar, which was extracted,vith DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent. No inhibitory effect was observed with a transport medium which did not contain agar.
引用
收藏
页码:275 / 276
页数:2
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