Application of the nucleotidylyltransferase Ep toward the chemoenzymatic synthesis of dTDP-desosamine analogues

被引：27

作者：

Jiang, JQ ^{[1
]}

Albermann, C ^{[1
]}

Thorson, JS ^{[1
]}

机构：

[1] Univ Wisconsin, Lab Biosynthet Chem, Pharmaceut Sci Div, Sch Pharm, Madison, WI 53705 USA

来源：

CHEMBIOCHEM | 2003年 / 4卷 / 05期

关键词：

antibiotics; enzyme catalysis; glycorandomization; glycosyltransferases; nucleotides;

D O I：

10.1002/cbic.200200566

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sweet transfer: The macrolide sugar β-D-desosamine (1) is a key contributor to the anti-infective properties of many macrolide antibiotics, and methods to alter this substituent may lead to macrolide variants with enhanced properties. The application of a nucleotidylyltransferase (Ep) toward the synthesis of analogues of the precursor dTDP-desosamine is demonstrated and the substrate specificity of this important class of enzyme and its integral participation in natural product 'glycorandomization' is commented on.

引用

页码：443 / 446

页数：4

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