High-performance liquid chromatographic fingerprint analysis for different origins of sea buckthorn berries

被引:96
作者
Chen, Chu [1 ]
Zhang, Hao [1 ]
Xiao, Wei [1 ]
Yong, Zheng-Ping [1 ]
Bai, Nan [1 ]
机构
[1] Sichuan Univ, W China Sch Pharm, Chengdu 610041, Peoples R China
基金
中国国家自然科学基金;
关键词
sea buckthorn; Hippophae; flavonoid; fingerprint; quantitative analysis; chemotaxonomy; HPLC;
D O I
10.1016/j.chroma.2007.03.097
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Using high-performance liquid chromatography (HPLC), a chemical fingerprint method was developed for investigating and demonstrating the variance of flavonoids among different origins of sea buckthorn berries. Thirty-four samples were analyzed including 15 RS (Hippophae rhamnoides ssp. sinensis) samples, 7 RY (H. rhamnoindes ssp. yunnanensis) samples, 5 RW (H. rhamnoides ssp. wolongensis) samples, 4 NS (H. neurocarpa ssp. stellatopilosa) samples and 3 TI (H. tibetana) samples. In the HPLC chromatograms, 12 compounds were identified as flavonoids, including quercetin 3-O-sophoroside-7-rhamnoside, kaempferol 3-O-sophoroside-7-O-rhamnoside, isorhamnetin 3-O-sophoroside7-O-rhamnoside, isorhamnetin 3-O-glucoside-7-O-rhamnoside, quercetin 3-O-rutinoside, quercetin 3-O-glucoside, isorhamnetin 3-O-rutinoside, isorhamnetin 3-O-glucoside, quercetin, kaempferol 7-O-rhamnoside, kaempferol and isorhamnetin. Both correlation coefficient of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. Our results revealed that the chromatographic fingerprint combining similarity evaluation could efficiently identify and distinguish sea buckthorn berries from different species. However, no obvious difference between RS and RY suggested that the two subspecies might have very close relationship in terms of chemotaxonomy. The established method was considered to be suitable for fingerprint analysis to check the genuine origin and control the quality of sea buckthorn berries and extracts. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:250 / 259
页数:10
相关论文
共 38 条
[1]   Sea buckthorn products: Manufacture and composition [J].
Beveridge, T ;
Li, TSC ;
Oomah, BD ;
Smith, A .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (09) :3480-3488
[2]  
[陈学林 Chen Xuelin], 2003, [西北植物学报, Acta Botanica Boreali-Occidentalla Sinica], V23, P451
[3]   Inhibitory effects of total flavones of Hippophae Rhamnoides L on thrombosis in mouse femoral artery and in vitro platelet aggregation [J].
Cheng, JY ;
Kondo, K ;
Suzuki, Y ;
Ikeda, Y ;
Meng, XS ;
Umemura, K .
LIFE SCIENCES, 2003, 72 (20) :2263-2271
[4]  
Duan J.A., 1999, J PLANT RESOURCE ENV, V8, P6
[5]   Effects of an antioxidant-rich juice (sea buckthorn) on risk factors for coronary heart disease in humans [J].
Eccleston, C ;
Baoru, Y ;
Tahvonen, R ;
Kallio, H ;
Rimbach, G ;
Minihane, AM .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2002, 13 (06) :346-354
[6]   Information theory applied to chromatographic fingerprint of herbal medicine for quality control [J].
Gong, F ;
Liang, YZ ;
Xie, PS ;
Chau, FT .
JOURNAL OF CHROMATOGRAPHY A, 2003, 1002 (1-2) :25-40
[7]   Comparison of high-speed counter-current chromatography and high-performance liquid chromatography on fingerprinting of Chinese traditional medicine [J].
Gu, M ;
Fan, OY ;
Su, ZG .
JOURNAL OF CHROMATOGRAPHY A, 2004, 1022 (1-2) :139-144
[8]   Hippophae rhamnoides L.:: chromatographic methods to determine chemical composition, use in traditional medicine and pharmacological effects [J].
Guliyev, VB ;
Gul, M ;
Yildirim, A .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 812 (1-2) :291-307
[9]   High-performance liquid chromatography with electrospray ionization mass spectrometry and diode array ultraviolet detection in the identification of flavonol aglycones and glycosides in berries [J].
Häkkinen, S ;
Auriola, S .
JOURNAL OF CHROMATOGRAPHY A, 1998, 829 (1-2) :91-100
[10]   Development, optimization and validation of a fingerprint of Ginkgo biloba extracts by high-performance liquid chromatography [J].
Ji, YB ;
Xu, QS ;
Hu, YZ ;
Heyden, YV .
JOURNAL OF CHROMATOGRAPHY A, 2005, 1066 (1-2) :97-104