Comparison of ribotyping and repetitive extragenic palindromic-PCR for identification of fecal Escherichia coli from humans and animals

被引:70
作者
Carson, CA
Shear, BL
Ellersieck, MR
Schnell, JD
机构
[1] Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA
[2] Univ Missouri, Agr Expt Stn Statisticians, Columbia, MO 65211 USA
关键词
D O I
10.1128/AEM.69.3.1836-1839.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This report compares the performances of two popular genotypic methods used for tracking the sources of fecal pollution in water, ribotyping and repetitive extragenic palindromic-PCR (rep-PCR). The rep-PCR was more accurate, reproducible, and efficient in associating DNA fingerprints of fecal Escherichia coli with human and animal hosts of origin.
引用
收藏
页码:1836 / 1839
页数:4
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