Conditional loss of PTEN leads to testicular teratoma and enhances embryonic germ cell production

被引:204
作者
Kimura, T [1 ]
Suzuki, A
Fujita, Y
Yomogida, K
Lomeli, H
Asada, N
Ikeuchi, M
Nagy, A
Mak, TW
Nakano, T
机构
[1] Osaka Univ, Res Inst Microbial Dis, Dept Mol Cell Biol, Osaka 5650871, Japan
[2] Akita Univ, Sch Med, Dept Biochem, Akita 0108543, Japan
[3] Osaka Univ, Res Inst Microbial Dis, Dept Lab Sci & Anim Experimentat, Osaka 5650871, Japan
[4] Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
[5] Ontario Canc Inst, Amgen Res Inst, Toronto, ON M5G 2C1, Canada
[6] Univ Toronto, Toronto, ON M5G 2C1, Canada
来源
DEVELOPMENT | 2003年 / 130卷 / 08期
关键词
PTEN; germ cells; teratoma; EG cells; mouse; human;
D O I
10.1242/dev.00392
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The tumor suppressor gene PTEN, which is frequently mutated in human cancers, encodes a lipid phosphatase for phosphatidylinositol 3,4,5-triphosphate [PtdIns(3,4,5)P-3] and antagonizes phosphatidylinositol 3 kinase. Primordial germ cells (PGCs), which are the embryonic precursors of gametes, are the source of testicular teratoma. To elucidate the intracellular signaling mechanisms that underlie germ cell differentiation and proliferation, we have generated mice with a PGC-specific deletion of the Pten gene. Male mice that lacked PTEN exhibited bilateral testicular teratoma, which resulted from impaired mitotic arrest and outgrowth of cells with immature characters. Experiments with PTEN-null PGCs in culture revealed that these cells had greater proliferative capacity and enhanced pluripotent embryonic germ (EG) cell colony formation. PTEN appears to be essential for germ cell differentiation and an important factor in testicular germ cell tumor formation.
引用
收藏
页码:1691 / 1700
页数:10
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