Enzyme-assisted calcium phosphate biomineralization on an inert alumina surface

被引：17

作者：

Aminian, Alieh ^{[1
]}

Pardun, Karoline ^{[1
]}

Volkmann, Eike ^{[1
]}

Li Destri, Giovanni ^{[2
]}

Marietta, Giovanni ^{[3
,4
]}

Treccani, Laura ^{[1
]}

Rezwan, Kurosch ^{[1
]}

机构：

[1] Univ Bremen, D-28359 Bremen, Germany

[2] ESRF European Synchrotron, F-38043 Grenoble 9, France

[3] Univ Catania, Dept Chem, Lab Mol Surfaces & Nanotechnol LAMSUN, I-95125 Catania, Italy

[4] CSGI, I-95125 Catania, Italy

来源：

ACTA BIOMATERIALIA | 2015年 / 13卷

关键词：

Alumina; Alkaline phosphatase; Surface functionalization; Biomineralization; Bioactivity; BOVINE SERUM-ALBUMIN; ALKALINE-PHOSPHATASE; MATRIX DEPOSITION; IN-VITRO; IMMOBILIZATION; CERAMICS; HYDROXYAPATITE; MINERALIZATION; ADSORPTION; SCAFFOLDS;

D O I：

10.1016/j.actbio.2014.11.007

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

In this study a bioinspired approach to induce self-mineralization of bone-like material on alumina surfaces is presented. The mineralizing enzyme alkaline phosphatase (ALP) is covalently immobilized by a carbodiimide-mediated chemoligation method. The enzymatic activity of immobilized ALP and its mineralization capability are investigated under acellular conditions as well as in the presence of human bone cells. Analytical, biochemical and immunohistochemical characterization show that ALP is efficiently immobilized, retains its activity and can trigger calcium phosphate mineralization on alumina at acellular conditions. In vitro cell tests demonstrate that ALP-functionalized alumina clearly boosts and enhances bone cell mineralization. Our results underpin the great potential of ALP-functionalized alumina for the development of bioactive surfaces for applications such as orthopaedic and dental implants, enabling a fast and firm implant osseointegration. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：335 / 343

页数：9

共 35 条

[1]

Alkaline phosphatase-induced mineral deposition to anchor collagen fibrils to a solid surface [J].