Behavior of human chondrocytes in engineered porous bacterial cellulose scaffolds

被引:80
作者
Andersson, Jessica [1 ]
Stenhamre, Hanna [1 ,2 ]
Backdahl, Henrik [1 ]
Gatenholm, Paul [1 ]
机构
[1] Chalmers Univ Technol, Dept Chem & Biol Engn, S-41296 Gothenburg, Sweden
[2] Univ Gothenburg, Sahlgrenska Univ Hosp, Dept Clin Chem & Transfus Med, S-41345 Gothenburg, Sweden
基金
瑞典研究理事会;
关键词
porous bacterial cellulose; scaffolds; human chondrocytes; cartilage regeneration; tissue engineering; ARTICULAR-CARTILAGE; MECHANICAL-PROPERTIES; MICROSPHERES; COLLAGEN;
D O I
10.1002/jbm.a.32784
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Regeneration of articular cartilage damage is an area of great interest due to the limited ability of cartilage to self-repair. The latest cartilage repair strategies are dependent on access to biomaterials to which chondrocytes can attach and in which they can migrate and proliferate, producing their own extracellular matrix. In the present study, engineered porous bacterial cellulose (BC) scaffolds were prepared by fermentation of Acetobacter xylinum (A. xylinum) in the presence of slightly fused wax particles with a diameter of 150-300 mu m, which were then removed by extrusion. This porous material was evaluated as a scaffold for cartilage regeneration. Articular chondrocytes from young adult patients as well as neonatal articular chondrocytes were seeded with various seeding techniques onto the porous BC scaffolds. Scanning electron microscopy (SEM) analysis and confocal microscopy analysis showed that cells entered the pores of the scaffolds and that they increasingly filled out the pores over time. Furthermore, DNA analysis implied that the chondrocytes proliferated within the porous BC. Alcian blue van Gieson staining revealed glycosaminoglycan (GAG) production by chondrocytes in areas where cells were clustered together. With some further development, this novel biomaterial can be a suitable candidate for cartilage regeneration applications. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 94A: 1124-1132, 2010.
引用
收藏
页码:1124 / 1132
页数:9
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