Simple method of isolation and purification of α-galactosides from legumes

被引：81

作者：

Gulewicz, P

Ciesiolka, D

Frias, J

Vidal-Valverde, C

Frejnagel, S

Trojanowska, K

Gulewicz, K

机构：

[1] Polish Acad Sci, Inst Bioorgan Chem, Phytochem Lab, PL-61704 Poznan, Poland

[2] Agr Univ Poznan, Dept Biotechnol & Food Microbiol, PL-60623 Poznan, Poland

[3] CSIC, Inst Fermentac Ind, E-28006 Madrid, Spain

[4] Polish Acad Sci, Inst Anim Reprod & Food Res, Div Food Sci, PL-10718 Olsztyn, Poland

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 08期

关键词：

alpha-galactosides; raffinose family oligosaccharides (RFOs); lentil; pea;

D O I：

10.1021/jf000210v

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A simple method for the isolation and purification of alpha-galactosides, raffinose family oligosaccharides (RFOs), from legumes has been developed. The method includes (i) imbibition of seeds, (ii) extraction with 50% ethanol, (iii) precipitation of RFOs, (iv) purification of RFOs on diatomaceous earth and charcoal, and (v) cation-exchange chromatography. The described method allows one to obtain high purity RFO preparations (90% for lentil and 80% for pea seeds, determined by HPLC-RI analysis) in the form of white, fine powder. Yields of alpha-galactosides isolated from 100 g of seeds of lentil and pea were 5.6 and 4.3 g, respectively.

引用

页码：3120 / 3123

页数：4

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