New isoflavones and pterocarpane with hepatoprotective activity from the stems of Erycibe expansa

被引：55

作者：

Matsuda, H ^{[1
]}

Morikawa, T ^{[1
]}

Xu, FM ^{[1
]}

Ninomiya, K ^{[1
]}

Yoshikawa, M ^{[1
]}

机构：

[1] Kyoto Pharmaceut Univ, Yamashina Ku, Kyoto 6078412, Japan

来源：

PLANTA MEDICA | 2004年 / 70卷 / 12期

关键词：

Erycibe expansa; Convolvulaceae; erycibenin; isoflavone; pterocarpane; hepatoprotective effect;

D O I：

10.1055/s-2004-835852

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The methanolic extract from the stems of Erycibe expansa was found to show a hepatoprotective effect on D-galactosamine-induced cytotoxicity in primary cultured mouse hepatocytes. By bioassay-guided separation, two new prenylisoflavones and a pterocarpane, erycibenins A (1), B (2), and C (3), were isolated from the active fraction (the EtOAc-soluble fraction) together with ten isoflavones (4-13) and seven pterocarpanes (14-20). The stereostructures of the new compounds were determined on the basis of chemical and physicochemical evidence including modified Mosher's method. In addition, the isolated constituents, erycibenin A (1, IC50 = 79 muM), genistein (6, 29 muM), orobol (7, 36 muM), and 5,7,4'-trihydroxy-3-methoxyisoflavone (8, 55 muM) exhibited inhibitory activity on D-galactosamine-induced cytotoxicity in primary cultured mouse hepatocytes.

引用

页码：1201 / 1209

页数：9

共 20 条

[1] Pterocarpans from Ononis viscosa subsp. breviflora [J].