Functional identification of the glycerol permease activity of Arabidopsis thaliana NLM1 and NLM2 proteins by heterologous expression in Saccharomyces cerevisiae

被引：73

作者：

Weig, AR ^{[1
]}

Jakob, C ^{[1
]}

机构：

[1] Univ Bayreuth, Inst Plant Physiol, D-95440 Bayreuth, Germany

来源：

FEBS LETTERS | 2000年 / 481卷 / 03期

关键词：

major intrinsic protein; aquaporin; aquaglyceroporin; water transport; glycerol transport; heterologous expression;

D O I：

10.1016/S0014-5793(00)02027-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NLM proteins (NOD26-like major intrinsic proteins) from plants contain amino acid sequence signatures which can be found in aquaporins including plant plasma membrane intrinsic proteins and tonoplast intrinsic proteins and glycerol permeases such as the Escherichia coli GlpF and the yeast FPSL proteins. Heterologous expression of two members of the NLM subgroup from Arabidopsis thaliana (AtNLM1 and AtNLM2) in baker's yeast demonstrated the glycerol permease activity in addition to the previously described aquaporin activity of AtNLM1. The transport mas non-saturable up to 100 mM extracellular glycerol concentration. Longer-chain sugar alcohols did not compete with the transport of radiolabelled glycerol and hexoses were also not transported through the pore. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：293 / 298

页数：6

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