Mau-2 acts cell-autonomously to guide axonal migrations in Caenorhabditis elegans

被引:21
作者
Bénard, CY [1 ]
Kébir, H [1 ]
Takagi, S [1 ]
Hekimi, S [1 ]
机构
[1] McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada
来源
DEVELOPMENT | 2004年 / 131卷 / 23期
关键词
mau-2; migration; guidance; slt-1; slit; Caenorhabditis elegans; maternal effect; cell autonomous;
D O I
10.1242/dev.01433
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The gene mau-2 has been found to be required for the guidance of cellular and axonal migrations along both the anteroposterior and the dorsoventral body axes during the development of the nematode C elegans. We show that mau-2 encodes a novel, previously uncharacterized protein that is highly conserved among animals. Maternal mau-2 gene expression is sufficient for normal development until the fourth larval stage, and a MAU-2::GFP fusion protein localizes to the cytoplasm of neurones. mau-2 is ubiquitously expressed in embryos by late gastrulation and becomes predominantly expressed in the nervous system as morphogenesis progresses. Expression of mau-2 within individual neurones rescues the guidance defects of mau-2 mutants, indicating that mau-2 functions cell-autonomously. Altering the activity of both the dorsal repellent slt-1 and mau-2 leads to the abnormal dorsal projection of the AVM axon, a phenotype that is novel and specific to the interaction of these two genes, indicating that mau-2 participates in the guidance of AVM by a slt-1-independent mechanism. Taken together, mau-2 defines a novel guidance factor that might be involved in the intracellular processing of guidance cues encountered by migrating cells and axons; during development.
引用
收藏
页码:5947 / 5958
页数:12
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