Supercritical CO2 oil extraction from Chinese star anise seed and simultaneous compositional analysis using HPLC by fluorescence detection and online atmospheric CI-MS identification

被引：8

作者：

Li, Guoliang ^{[1
,2
]}

Sun, Zhiwei ^{[1
,2
]}

Xia, Lian ^{[1
,2
]}

Shi, Junyou ^{[1
,2
]}

Liu, Yongjun ^{[1
]}

Suo, Yourui ^{[1
]}

You, Jinmao ^{[1
]}

机构：

[1] Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 2010年 / 90卷 / 11期

基金：

美国国家科学基金会;

关键词：

Chinese star anise seed oil; supercritical CO2 fluid extraction; response surface methodology; fatty acids; HPLC/APCI/MS; FATTY-ACIDS; OPTIMIZATION; POLLEN;

D O I：

10.1002/jsfa.4031

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

BACKGROUND: Supercritical CO2 was utilised to extract Chinese star anise seed oil (CSASO), and a three-level Box - Behnken factorial design from response surface methodology was applied to optimise the extraction conditions, including pressure, temperature and amount of modifier (ethanol). The compositional analysis of fatty acids in CSASO was performed by HPLC with fluorescence detection using 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methylbenzenesulfonate (BCETS) as labelling reagent. Identification was carried out by online atmospheric chemical ionisation-mass spectrometry. RESULTS: The optimum extraction conditions were as follows: extraction pressure, 27.72 MPa, extraction temperature, 46.22 degrees C, and amount of modifier, 8.58 vol.%. The experimental result showed that the maximum extraction yield was 25.31 +/- 0.22% (w/w) under the conditions proposed. The compositional analysis indicated that CSASO mainly contained C18: 2, C18 : 1, C18 : 3, C20 :4, C16, C18 and C20 fatty acids. CONCLUSION: In this study, a fast, simple and high-efficiency supercritical technique for extracting oil from Chinese star anise seed was developed. Simultaneous determination of fatty acids in CSASO using BCETS as the labelling reagent with HPLC fluorescence detection and online mass spectroscopy identification has been successfully achieved. (C) 2010 Society of Chemical Industry

引用

页码：1905 / 1913

页数：9

共 27 条

[1]

Extraction of essential oils from the seeds of pomegranate using organic solvents and supercritical CO2 [J].