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Probing ligand-protein recognition with sum-frequency generation spectroscopy:: The avidin-biocytin case

被引：40
作者：
Dreesen, L
Sartenaer, Y
Humbert, C
Mani, AA
Méthivier, C
Pradier, CM
Thiry, PA
Peremans, A
机构：
[1] Univ Namur, Lab Spect Mol Surface, B-5000 Namur, Belgium
[2] Ecole Natl Super Chim Paris, CNRS, Lab Phys Chim Surfaces, UMR 7045, F-75231 Paris 05, France
来源：
CHEMPHYSCHEM | 2004年 / 5卷 / 11期
关键词：
biosensors; laser spectroscopy; molecular recognition; monolayers; vibrational spectroscopy;
D O I：
10.1002/cphc.200400213
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the some spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.
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收藏
页码：1719 / 1725
页数：7
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