A new efficient expression system for Bacillus and its application to production of recombinant phytase

被引：26

作者：

Kerovuo, J ^{[1
]}

von Weymarn, N

Povelainen, M

Auer, S

Miasnikov, A

机构：

[1] Danisco Cultor Innovat, Kantvik Res Ctr, Kantvik, Finland

[2] Helsinki Univ Technol, Lab Bioproc Engn, FIN-02150 Espoo, Finland

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 16期

关键词：

Bacillus subtilis; fed-batch cultivation; phosphate-starvation; phytase; plasmid;

D O I：

10.1023/A:1005694731039

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new expression system was developed for Bacillus subtilis. This system uses a shuttle vector (B. subtilis - Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillus phytase - phytase activity at the end of cultivation reached 28.7 U ml(-1).

引用

页码：1311 / 1317

页数：7

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