Application of a sensitive catechol detector for determination of tyrosinase inhibitors

被引:37
作者
Streffer, K
Kaatz, H
Bauer, CG
Makower, A
Schulmeister, T
Scheller, FW
Peter, MG
Wollenberger, U
机构
[1] Max Delbruck Ctr Mol Med, Univ Potsdam, Inst Biochem & Mol Physiol, D-13122 Berlin, Germany
[2] Univ Potsdam, Inst Organ Chem & Struct Anal, D-14469 Potsdam, Germany
[3] Univ Potsdam, Dept Bioinformat, D-14469 Potsdam, Germany
关键词
tyrosinase; glucose dehydrogenase; inhibition; carboxylic acid; kojic acid; thiourea derivatives;
D O I
10.1016/S0003-2670(97)00690-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A substrate regenerating bienzyme sensor was used to measure a variety of organic and inorganic inhibitors with high sensitivity. The bienzyme system consists of the two cooperating enzymes, cytosolic quinoprotein glucose dehydrogenase and mushroom tyrosinase. The cooperation takes place on the substrate/product level. Under kinetic control, compounds that affect one of the enzymes can be detected with high sensitivity. For the construction of the sensor, the enzymes were immobilized in polyvinyl alcohol, coupled to a Clark-type oxygen electrode and the oxygen consumption was monitored Curing catechol conversion. In this way, carboxylic acids, kojic acids, inorganic ions and thiourea derivatives were studied. Theoretical considerations reveal the relationship between amplification gain and inhibitor concentration. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:81 / 90
页数:10
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