Silicone-protein films: Establishing the strength of the protein-silicone interaction

被引：12

作者：

Bartzoka, V

Brook, MA

McDermott, MR

机构：

[1] McMaster Univ, Dept Chem, Hamilton, ON L8S 4M1, Canada

[2] McMaster Univ, Dept Pathol, Hamilton, ON L8N 3Z5, Canada

来源：

LANGMUIR | 1998年 / 14卷 / 07期

关键词：

D O I：

10.1021/la9711138

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Protein-on-silicone and silicone-on-protein films were prepared using either trimethylsilyl-terminated (unfunctionalized, PDMS) or (triethoxysilyl)propyl-terminated (functionalized, TES-PDMS) silicones and human serum albumin (HSA). The films were exposed to aqueous solutions of different pH's or containing SDS. X-ray photoelectron spectroscopy (XPS) and contact-angle measurements (CA) were used to characterize the resulting surfaces. The film formed by coating a protein surface with TES-PDMS showed much slower protein desorption kinetics than the other slides upon challenge by SDS, acidic, neutral, or basic aqueous solutions. This observation may be understood to arise from the cross-linking of TES-PDMS using the protein as a template, resulting in intimate contact between the two polymers.

引用

页码：1892 / 1898

页数：7

共 39 条

[11]

COLVIN EW, 1981, SILICON ORGNAIC SYNT

[12]

COOPER SL, 1995, ACS SYM SER, V602, P2

[13]

EABORN C, 1960, ORGANOSILICON COMPOU, P295

[14]

ERTEL SI, 1991, J COLLOID INTERF SCI, V133, P148

[15]

EVANS D, 1994, COMPUTER WEEKLY 0428, P6

[16]

Fowkes M., 1964, CONTACT ANGLE WETTAB

[17]

FRANSSEN EJF, 1996, PROGR MED CHEM, V1, P355

[18] GLOBULAR-PROTEINS AT SOLID-LIQUID INTERFACES [J].

HAYNES, CA ;

NORDE, W .

COLLOIDS AND SURFACES B-BIOINTERFACES, 1994, 2 (06) :517-566

[19] Novel polymer-grafted starch microparticles for mucosal delivery of vaccines [J].

Heritage, PL ;

Loomes, LM ;

Jianxiong, J ;

Brook, MA ;

Underdown, BJ ;

McDermott, MR .

IMMUNOLOGY, 1996, 88 (01) :162-168

[20]

HORBETT TA, 1987, ACS SYM SER, V343, P1

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