Effects of caveolin-1 on the 17β-estradiol-mediated inhibition of VSMC proliferation induced by vascular injury

被引：13

作者：

Liu, Hai-Mei ^{[1
]}

Zhao, Xiao-Feng ^{[1
]}

Guo, Liao-Nan ^{[1
]}

Tan, Zhi ^{[1
]}

Wang, Ting-Huai ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Dept Physiol, Zhongshan Med Coll, Guangzhou 510080, Peoples R China

来源：

LIFE SCIENCES | 2007年 / 80卷 / 08期

基金：

中国国家自然科学基金;

关键词：

vascular smooth muscle cell; caveolin-1; 17; beta-estradiol; vascular injury;

D O I：

10.1016/j.lfs.2006.11.014

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

Estrogen has a protective effect on the cardiovascular system. Yet the mechanism of how estrogen inhibits vascular smooth muscle cell (VSMC) proliferation after vascular injury and the role of caveolin-1 in this process are not clear. To understand the protection effect of estrogen and caveolin-1, we employed a vascular balloon-injury model. Sixteen New Zealand White rabbits with or without estrogen were tested. 17 beta-estradiol is able to inhibit VSMC proliferation in a range from 10(-10)-10(-5) mol/L, with an optimal concentration of 10(-8) mol/L. Estrogen exerted its effect through suppressing the activity of p42/44 MAPK, which can be blocked by tamoxifen. Moreover, in estrogen pretreated cells as well as in common carotid arteries of the balloon injury model, expression of caveolin-1 is enhanced compared to the estrogen-deficient group, as assessed by both western blotting and RT-PCR and morphological studies. Our results showed that the inhibition effect of estrogen in VSMCs is mediated by p42/44 MAPK. Caveolin-1 plays an important role in this protective process. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：800 / 812

页数：13

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