On-line size-exclusion chromatography/mass spectrometry of low molecular mass heparin

被引：47

作者：

Henriksen, J

Ringborg, LH

Roepstorrf, P

机构：

[1] Leo Pharmaceut Prod, Dept Spect, DK-2750 Ballerup, Denmark

[2] Univ So Denmark, Dept Mol Biol, DK-5230 Odense, Denmark

来源：

JOURNAL OF MASS SPECTROMETRY | 2004年 / 39卷 / 11期

关键词：

low molecular mass heparin; electrospray ionization; size-exclusion chromatography; time-of-flight mass spectrometry; on-line cation exchange;

D O I：

10.1002/jms.723

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Heparin and low molecular mass heparin (LMMH) consists of complex mixtures of sulphated linear oligosaccharides that are difficult to analyse. An on-line size exclusion chromatographic/electrospray ionization (ESI) mass spectrometric method that allows the determination of more than 60 components in an LMMH preparation is presented. The experimental setup includes on-line cation exchange in order to prevent massive adducting in the ESI interface. Copyright (C) 2004 John Wiley Sons, Ltd.

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收藏

页码：1305 / 1312

页数：8

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