Thiazolidinediones downregulate stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes

被引：43

作者：

Kurebayashi, S ^{[1
]}

Hirose, T ^{[1
]}

Miyashita, Y ^{[1
]}

Kasayama, S ^{[1
]}

Kishimoto, T ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT MED 3,SUITA,OSAKA 565,JAPAN

来源：

DIABETES | 1997年 / 46卷 / 12期

关键词：

D O I：

10.2337/diabetes.46.12.2115

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Thiazolidinediones (TZDs) are known to have potent increases of insulin sensitivity, Because peroxisome proliferator-activated receptor-gamma (PPAR-gamma), a receptor for TZDs, is mainly expressed in adipocytes, we tried to search the TZD-targeted genes in mouse 3T3-L1 adipocytes, By the mRNA differential display method, one band repressed by troglitazone was obtained, which corresponded to the partial sequences of the stearoyl-CoA desaturase 1 (SCD1) gene, Troglitazone dramatically decreased SCD1 mRNA levels in 3T3-L1 adipocytes in a dose-dependent manner. Pioglitazone also repressed the SCD1 mRNA expression, whereas WY-14,643 had no apparent effect. Both troglitazone and pioglitazone raised the composition (weight percentage) of myristic acid (C14:0), palmitic acid (C16:0), and stearic acid (C18:0), but lowered the composition of the Delta(9)-cis desaturated fatty acids such as myristoleic acid (C14:1, Delta(9)), palmitoleic acid (C16:1 Delta(9)), oleic acid (C14:1, Delta(9)), and linoleic acid (C18:2, Delta(9), (12)). These results indicate that TZDs repress SCD1 activity in 3T3-L1 adipocytes via downregulating SCD1 enzyme gene expression.

引用

页码：2115 / 2118

页数：4

共 25 条

[1] Differential activation of adipogenesis by multiple PPAR isoforms [J].