Properties of polyclonal, monoclonal, and recombinant antibodies recognizing the organophosphorus pesticide chlorpyrifos-ethyl

被引：36

作者：

Alcocer, MJC ^{[1
]}

Doyen, C ^{[1
]}

Lee, HA ^{[1
]}

Morgan, MRA ^{[1
]}

机构：

[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 09期

关键词：

variable region; antibody engineering; immunoassay; recombinant antibody; pesticide analysis;

D O I：

10.1021/jf990917l

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A rabbit polyclonal antiserum and two murine monoclonal antibodies recognizing the organophosphorus pesticide chlorpyrifos-ethyl were produced. The two hybridoma cell lines were then used as sources of immunoglobulin genes for the generation of recombinant scFv antibodies in Escherichia coli. The two scFvs showed either similar or improved limits of detection in an ELISA when compared with the monoclonal antibodies. Cross-reactivity studies showed that all of the antibodies were specific toward the chlorinated aromatic ring. Furthermore, scFv gene sequences were linked directly to sequences coding for either a c-Myc tag, a His-tag, or alkaline phosphatase; The fusion products generated were functional, and their properties were determined. The problems associated with producing scFvs and scFv derivatives for detection of pesticide residues from hybridoma are addressed and discussed.

引用

页码：4053 / 4059

页数：7

共 45 条

[1] [Anonymous], 1995, OFF METH AN, V16th
[2] BELL CW, 1995, GENE, V165, P323, DOI 10.1016/0378-1119(95)00531-A
[3] Cloning, expression and characterization of a single-chain antibody specific for the herbicide atrazine
Byrne, FR
Grant, SD
Porter, AJ
Harris, WJ
[J]. FOOD AND AGRICULTURAL IMMUNOLOGY, 1996, 8 (01) : 19 - 29
[4] HYBRIDOMA FUSION CELL-LINES CONTAIN AN ABERRANT KAPPA TRANSCRIPT
CARROLL, WL
MENDEL, E
LEVY, S
[J]. MOLECULAR IMMUNOLOGY, 1988, 25 (10) : 991 - 995
[5] CHLORPYRIFOS - HAZARD ASSESSMENT BASED ON A REVIEW OF THE EFFECTS OF SHORT-TERM AND LONG-TERM EXPOSURE IN ANIMALS AND HUMANS
COCHRAN, RC
KISHIYAMA, J
ALDOUS, C
CARR, WC
PFEIFER, KF
[J]. FOOD AND CHEMICAL TOXICOLOGY, 1995, 33 (02) : 165 - 172
[6] A NOVEL STRATEGY FOR THE IMMUNOLOGICAL TAGGING OF CDNA CONSTRUCTS
CRAVCHIK, A
MATUS, A
[J]. GENE, 1993, 137 (01) : 139 - 143
[7] The effect of the human serum paraoxonase polymorphism is reversed with diazoxon, soman and sarin
Davies, HG
Richter, RJ
Keifer, M
Broomfield, CA
Sowalla, J
Furlong, CE
[J]. NATURE GENETICS, 1996, 14 (03) : 334 - 336
[8] ELIMINATION OF ENDOGENOUS ABERRANT KAPPA-CHAIN TRANSCRIPTS FROM SP2/0-DERIVED HYBRIDOMA CELLS BY SPECIFIC RIBOZYME CLEAVAGE - UTILITY IN GENETIC THERAPY OF HIV-1 INFECTIONS
DUAN, LX
POMERANTZ, RJ
[J]. NUCLEIC ACIDS RESEARCH, 1994, 22 (24) : 5433 - 5438
[9] Edward Simone L., 1993, Food and Agricultural Immunology, V5, P129, DOI 10.1080/09540109309354792
[10] [FDA] US Food and Drug Administration, 1994, J AOAC INT, V77, p161A, DOI 10.1093/jaoac/77.5.161A

← 1 2 3 4 5 →