Properties of polyclonal, monoclonal, and recombinant antibodies recognizing the organophosphorus pesticide chlorpyrifos-ethyl

被引：37

作者：

Alcocer, MJC ^{[1
]}

Doyen, C ^{[1
]}

Lee, HA ^{[1
]}

Morgan, MRA ^{[1
]}

机构：

[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 09期

关键词：

variable region; antibody engineering; immunoassay; recombinant antibody; pesticide analysis;

D O I：

10.1021/jf990917l

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A rabbit polyclonal antiserum and two murine monoclonal antibodies recognizing the organophosphorus pesticide chlorpyrifos-ethyl were produced. The two hybridoma cell lines were then used as sources of immunoglobulin genes for the generation of recombinant scFv antibodies in Escherichia coli. The two scFvs showed either similar or improved limits of detection in an ELISA when compared with the monoclonal antibodies. Cross-reactivity studies showed that all of the antibodies were specific toward the chlorinated aromatic ring. Furthermore, scFv gene sequences were linked directly to sequences coding for either a c-Myc tag, a His-tag, or alkaline phosphatase; The fusion products generated were functional, and their properties were determined. The problems associated with producing scFvs and scFv derivatives for detection of pesticide residues from hybridoma are addressed and discussed.

引用

页码：4053 / 4059

页数：7

共 45 条

[41] Mice lacking serum paraoxonase are susceptible to organophosphate toxicity and atherosclerosis [J].