The genomic structure of the chicken ICSBP gene and its transcriptional regulation by chicken interferon

被引：11

作者：

Dosch, E

Zöller, B

Müller-Redmann, I

Nanda, I

Schmid, M

Viciano-Gofferge, A

Jungwirth, C

机构：

[1] Univ Wurzburg, Inst Virol & Immunbiol, D-97078 Wurzburg, Germany

[2] Univ Wurzburg, Inst Human Genet, D-97074 Wurzburg, Germany

来源：

GENE | 1998年 / 210卷 / 02期

关键词：

evolution; avian; DNA-binding protein; interferon regulatory factor;

D O I：

10.1016/S0378-1119(98)00063-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The chicken interferon consensus sequence binding protein (ChICSBP) gene spans over 9 kb of DNA and consists, as its murine homolog, of nine exons. The first untranslated exon was identified by 5'-RACE technology. The second exon contains the translation initiation codon. Canonical consensus splice sites are found on every exon/intron junction. The introns are generally smaller than their mammalian counterparts. The ChICSBP and ChIRF-1 genes have been mapped by fluorescence in situ hybridization to different microchromosomes. The transcription start site has been mapped by primer extension. Inspection of the DNA sequence of a genomic clone containing the first exon and the region 1700-bp upstream revealed several potential cis-regulatory elements of transcription. The ChICSBP mRNA is induced by recombinant ChIFN type I and ChIFN-gamma. A palindromic IFN regulatory element (pIRE) with high sequence homology to gamma activation site (GAS) sequences was functionally required in transient transfection assays for the induction of transcription by ChIFN-gamma. (C) 1998 Elsevier Science B.V.

引用

页码：265 / 275

页数：11