Development of a real-time RT-PCR assay for a novel influenza A (H1N1) virus

被引：22

作者：

Jiang, Tao ^{[1
]}

Kang, XiaoPing ^{[1
]}

Deng, Yongqiang ^{[1
]}

Zhao, Hui ^{[1
]}

Li, Xiaofeng ^{[1
]}

Yu, Xuedong ^{[1
]}

Yu, Man ^{[1
]}

Qin, Ede ^{[1
]}

Zhu, Qingyu ^{[1
]}

Yang, Yinhui ^{[1
]}

Qin, Chengfeng ^{[1
]}

机构：

[1] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 163卷 / 02期

关键词：

Pandemic; Influenza A virus; H1N1; Real-time RT-PCR; TRANSMISSION; PATHOGENESIS;

D O I：

10.1016/j.jviromet.2009.09.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A pandemic caused by a novel influenza A virus (H1N1) poses a serious public health threat. In this study, a real-time reverse transcriptase PCR (RT-PCR) assay based on the hemagglutinin gene was developed that discriminates the novel H1N1 from swine influenza virus, seasonal H1N1/H3N2 virus and the highly pathogenic H5N1 avian influenza virus. The sensitivity of this assay was 0.2 50% tissue culture infective dose of virus and 200 copies of in vitro-transcribed target RNA. Three hundred and forty-eight clinical specimens from suspected H1N1 patients were tested using this assay, and forty-two (12.07%) were found to be positive. Tests using the real-time PCR assay recommended by WHO and virus isolation gave identical results. This sensitive and specific real-time RT-PCR assay will contribute to the early diagnosis and control of the emerging H1N1 influenza pandemic. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：470 / 473

页数：4

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