High expression of a human lactoferrin in transgenic tobacco cell cultures

被引：29

作者：

Choi, SM

Lee, OS

Kwon, SY

Kwak, SS

Yu, DY

Lee, HS

机构：

[1] KRIBB, Lab Plant Cell Biotechnol, Taejon 305806, South Korea

[2] KRIBB, Lab Environm Biotechnol, Taejon 305806, South Korea

[3] KRIBB, Lab Dev & Differentiat, Taejon 305806, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 03期

关键词：

high expression promoter; human lactoferrin; recombinant protein; transgenic plant cell culture;

D O I：

10.1023/A:1022341917735

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transgenic Nicotiana tabacum cell lines were developed expressing the human lactoferrin gene driven by the oxidative stress-inducible peroxidase (SWPA2) promoter. Western blot analysis showed the accumulation of both the full-length human lactoferrin protein as well as a immuno-reactive truncated fragment. Accumulation of human lactoferrin as monitored by ELISA increased proportionally to cell growth and reached a maximal (up to 4.3% of total soluble proteins) at the stationary phase of growth. Protein extracts from transgenic tobacco cells exhibited antibacterial activity.

引用

页码：213 / 218

页数：6

共 18 条

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[2]

Arakawa T, 1999, ADV EXP MED BIOL, V464, P149

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