Rutaecarpine inhibits angiotensin II-induced proliferation in rat vascular smooth muscle cells

被引:18
作者
Li Yan-ju [1 ,2 ,3 ]
Zhang Feng [1 ,2 ]
Gong Qi-hai [1 ,2 ]
Wu Qin [1 ,2 ]
Yu Li-mei [1 ,2 ]
Sun An-sheng [1 ,2 ]
机构
[1] Zunyi Med Coll, Key Lab Basic Pharmacol Guizhou, Zunyi 563000, Guizhou Provinc, Peoples R China
[2] Zunyi Med Coll, Dept Pharmacol, Zunyi 563000, Guizhou Provinc, Peoples R China
[3] Guiyang Med Coll, Affiliated Hosp, Dept Hematol, Guiyang 550004, Guizhou Provinc, Peoples R China
基金
中国国家自然科学基金;
关键词
rutaecarpine; angiotensin II; nitric oxide; vascular smooth muscle cell proliferation; gene expression; NITRIC-OXIDE; MECHANISMS; EXPRESSION; CALCIUM;
D O I
10.1007/s11655-013-1198-4
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
To evaluate the effects and possible mechanisms of rutaecarpine on angiotensin II (Ang II)-induced proliferation in cultured rat vascular smooth muscle cells (VSMCs). VSMCs were isolated from Male Sprague-Dawley rat aorta, and cultured by enzymic dispersion method. Experiments were performed with cells from passages 3-8. The cultured VSMCs were randomly divided into control, model (Ang II 0.1 mu mol/L), and rutaecarpine (0.3-3.0 mu mol/L) groups. VMSC proliferation was induced by Ang II, and was evaluated by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay and cell counting. To examine the mechanisms involved in anti-proliferative effects of rutaecarpine, nitric oxide (NO) levels and NO synthetase (NOS) activity were determined. Expressions of VSMC proliferation-related genes including endothelial nitric oxide synthase (eNOS), and c-myc hypertension related gene-1 (HRG-1) were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR). Rutaecarpine (0.3-3.0 mu mol/L) inhibited Ang II-induced VSMC proliferation and the best effects were achieved at 3.0 mu mol/L. The Ang II-induced decreases in cellular NO contents and NOS activities were antagonized by rutaecarpine (P < 0.05). Ang II administration suppressed the expressions of eNOS and HRG-1, while increased c-myc expression (P < 0.05). All these effects were attenuated by 3.0 mu mol/L rutaecarpine (P < 0.05). Rutaecarpine is effective against Ang II-induced rat VSMC proliferation, and this effect is due, at least in part, to NO production and the modulation of VMSC proliferation-related gene expressions.
引用
收藏
页码:682 / 687
页数:6
相关论文
共 28 条
[1]   Regulation of L-type inward calcium channel activity by captopril and angiotensin II via the phosphatidyl inositol 3-kinase pathway in cardiomyocytes from volume-overload hypertrophied rat hearts [J].
Alvin, Zikiar ;
Laurence, Graham G. ;
Coleman, Bernell R. ;
Zhao, Aiqiu ;
Hajj-Moussa, Majd ;
Haddad, Georges E. .
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2011, 89 (03) :206-215
[2]   Rutaecarpine prevents hypoxia-reoxygenation-induced myocardial cell apoptosis via inhibition of NADPH oxidases [J].
Bao, Mei-Hua ;
Dai, Wen ;
Li, Yuan-Jian ;
Hu, Chang-Ping .
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2011, 89 (03) :177-186
[3]   An in situ evidence for autocrine function of NO in the vasculature [J].
Buchwalow, IB ;
Podzuweit, T ;
Samoilova, VW ;
Wellner, M ;
Haller, H ;
Grote, S ;
Aleth, S ;
Boecker, W ;
Schmitz, W ;
Neumann, J .
NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 2004, 10 (04) :203-212
[4]   Vascular smooth muscle and nitric oxide synthase [J].
Buchwalow, IB ;
Podzuweit, T ;
Böcker, W ;
Samoilova, VE ;
Thomas, S ;
Wellnr, M ;
Baba, HA ;
Robenek, H ;
Schnekenburger, J ;
Lerch, MM .
FASEB JOURNAL, 2002, 16 (06) :500-508
[5]   RETRACTED: 6-Mercaptopurine reverses experimental vasospasm and alleviates the production of endothelins in NO-independent mechanism-a laboratory study (Retracted Article) [J].
Chang, Chih-Zen ;
Wu, Shu-Chuan ;
Kwan, Aij-Lie ;
Lin, Chih-Long ;
Hwang, Shiuh-Lin .
ACTA NEUROCHIRURGICA, 2011, 153 (04) :939-949
[6]   Comparative study on the vasodilatory effects of three quinazoline alkaloids isolated from Evodia rutaecarpa [J].
Chiou, WF ;
Liao, JF ;
Chen, CF .
JOURNAL OF NATURAL PRODUCTS, 1996, 59 (04) :374-378
[7]  
Cohen JD, 2007, J MANAGE CARE PHARM, V13, pS6
[8]   Stimulation of calcitonin gene-related peptide synthesis and release: mechanisms for a novel anti hypertensive drug, rutaecarpine [J].
Deng, PY ;
Ye, F ;
Cai, WJ ;
Tan, GS ;
Hu, CP ;
Deng, HW ;
Li, YJ .
JOURNAL OF HYPERTENSION, 2004, 22 (09) :1819-1829
[9]   Resveratrol inhibits rat aortic vascular smooth muscle cell proliferation via estrogen receptor dependent nitric oxide production [J].
Ekshyyan, Viktoriya P. ;
Hebert, Valeria Y. ;
Khandelwal, Alok ;
Dugas, Tammy R. .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2007, 50 (01) :83-93
[10]   NPRA-mediated suppression of AngII-induced ROS production contribute to the antiproliferative effects of B-type natriuretic peptide in VSMC [J].
Gao, Pan ;
Qian, De-Hui ;
Li, Wei ;
Huang, Lan .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2009, 324 (1-2) :165-172