Spiral phase contrast imaging in microscopy

被引：572

作者：

Fürhapter, S ^{[1
]}

Jesacher, A ^{[1
]}

Bernet, S ^{[1
]}

Ritsch-Marte, M ^{[1
]}

机构：

[1] Innsbruck Med Univ, Div Biomed Phys, A-6020 Innsbruck, Austria

来源：

OPTICS EXPRESS | 2005年 / 13卷 / 03期

关键词：

D O I：

10.1364/OPEX.13.000689

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate an optical method for edge contrast enhancement in light microscopy. The method is based on holographic Fourier plane filtering of the microscopic image with a spiral phase element (also called vortex phase or helical phase filter) displayed as an off-axis hologram at a computer controlled high resolution spatial light modulator (SLM) in the optical imaging pathway. The phase hologram imprints a helical phase term of the form exp(iphi) on the diffracted light field in its Fourier plane. In the image plane, this results in a strong and isotropic edge contrast enhancement for both amplitude and phase objects. (C) 2005 Optical Society of America.

引用

页码：689 / 694

页数：6

共 11 条

[1] Linear phase imaging using differential interference contrast microscopy [J].

Arnison, MR ;

Larkin, KG ;

Sheppard, CJR ;

Smith, NI ;

Cogswell, CJ .

JOURNAL OF MICROSCOPY-OXFORD, 2004, 214 (01) :7-12

[2] Optical processing with vortex-producing lenses [J].

Crabtree, K ;

Davis, JA ;

Moreno, I .

APPLIED OPTICS, 2004, 43 (06) :1360-1367

[3] Image processing with the radial Hilbert transform: theory and experiments [J].

Davis, JA ;

McNamara, DE ;

Cottrell, DM ;

Campos, J .

OPTICS LETTERS, 2000, 25 (02) :99-101

[4] GENERATION OF OPTICAL-PHASE SINGULARITIES BY COMPUTER-GENERATED HOLOGRAMS [J].

HECKENBERG, NR ;

MCDUFF, R ;

SMITH, CP ;

WHITE, AG .

OPTICS LETTERS, 1992, 17 (03) :221-223

[5] Diffractive optical tweezers in the Fresnel regime [J].

Jesacher, A ;

Fürhapter, S ;

Bernet, S ;

Ritsch-Marte, M .

OPTICS EXPRESS, 2004, 12 (10) :2243-2250

[6] Natural demodulation of two-dimensional fringe patterns. I. General background of the spiral phase quadrature transform [J].

Larkin, KG ;

Bone, DJ ;

Oldfield, MA .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2001, 18 (08) :1862-1870

[7] Quantitative optical microscope with enhanced resolution using a pixelated liquid crystal spatial light modulator [J].

Ng, AYM ;

See, CW ;

Somekh, MG .

JOURNAL OF MICROSCOPY, 2004, 214 :334-340

[8] Production and characterization of spiral phase plates for optical wavelengths [J].

Oemrawsingh, SSR ;

van Houwelingen, JAW ;

Eliel, ER ;

Woerdman, JP ;

Verstegen, EJK ;

Kloosterboer, JG ;

't Hooft, GW .

APPLIED OPTICS, 2004, 43 (03) :688-694

[9] Transverse mode shaping and selection in laser resonators [J].

Oron, Ram ;

Davidson, Nir ;

Friesem, Asher A. ;

Hasman, Erez .

PROGRESS IN OPTICS, VOL 42, 2001, 42 :325-386

[10]

REYNOLD SGO, 1989, NEW PHYS OPTICS NOTE

← 1 2 →