Development and validation of a method for the quantitation of Δ9-tetrahydrocannabinol in oral fluid by liquid chromatography electrospray-mass-spectrometry

被引：39

作者：

Concheiro, M ^{[1
]}

de Castro, A ^{[1
]}

Quintela, O ^{[1
]}

Cruz, A ^{[1
]}

López-Rivadulla, M ^{[1
]}

机构：

[1] Univ Santiago Compostela, Fac Med, Forens Toxicol Serv, Inst Legal Med, Santiago De Compostela 15786, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 810卷 / 02期

关键词：

Delta(9)tetrahydrocannabinol; oral fluid; LC-MS;

D O I：

10.1016/j.jchromb.2004.08.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of Delta(9)tetrahydrocannabinol (Delta(9)THC) and its metabolites in biological samples is of great relevance for forensic purposes. In the case of oral fluid (OF), the analysis should determine Delta(9)THC, whereas in urine, it detects the inactive metabolite tetrahydrocannabinol carboxylic acid (THC-COOH). Most laboratories analyze Delta(9)THC in such samples using GC-MS methods, but these procedures are time-consuming and involve unavoidable previous extraction and derivatization. No data is yet available on the application of liquid chromatography-mass-spectrometry to detect Delta(9)THC in oral fluid. We report a validation method in which the Delta(9)THC is isolated from oral fluid by a simple liquid-liquid extraction with hexane and subsequently analyzed by liquid chromatography-mass-spectrometry. The method here reported for the determination of Delta(9)THC in oral fluid only requires 200 mul of sample and achieves limits of detection of 2 ng/ml, and has been used to analyze oral fluid samples collected from current drug users. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：319 / 324

页数：6

共 26 条

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