Death don't have no mercy and neither does calcium:: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and innate immunity

被引:277
作者
Ali, Rashid [1 ]
Ma, Wei [1 ]
Lemtiri-Chlieh, Fouad [1 ]
Tsaltas, Dimitrios [1 ]
Leng, Qiang [1 ]
von Bodman, Susannne [1 ]
Berkowitz, Gerald A. [1 ]
机构
[1] Univ Connecticut, Agr Biotechnol Lab, Storrs, CT 06269 USA
关键词
D O I
10.1105/tpc.106.045096
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plant innate immune response to pathogen infection includes an elegant signaling pathway leading to reactive oxygen species generation and resulting hypersensitive response (HR); localized programmed cell death in tissue surrounding the initial infection site limits pathogen spread. A veritable symphony of cytosolic signaling molecules (including Ca2+, nitric oxide [NO], cyclic nucleotides, and calmodulin) have been suggested as early components of HR signaling. However, specific interactions among these cytosolic secondary messengers and their roles in the signal cascade are still unclear. Here, we report some aspects of how plants translate perception of a pathogen into a signal cascade leading to an innate immune response. We show that Arabidopsis thaliana CYCLIC NUCLEOTIDE GATED CHANNEL2 (CNGC2/DND1) conducts Ca2+ into cells and provide a model linking this Ca2+ current to downstream NO production. NO is a critical signaling molecule invoking plant innate immune response to pathogens. Plants without functional CNGC2 lack this cell membrane Ca2+ current and do not display HR; providing the mutant with NO complements this phenotype. The bacterial pathogen associated molecular pattern elicitor lipopolysaccharide activates a CNGC Ca2+ current, which may be linked to NO generation due to buildup of cytosolic Ca2+/calmodulin.
引用
收藏
页码:1081 / 1095
页数:15
相关论文
共 69 条
[21]   Hyperpolarization-activated Ca2+-permeable channels in the plasma membrane of tomato cells [J].
Gelli, A ;
Blumwald, E .
JOURNAL OF MEMBRANE BIOLOGY, 1997, 155 (01) :35-45
[22]   The RPM1 plant disease resistance gene facilitates a rapid and sustained increase in cytosolic calcium that is necessary for the oxidative burst and hypersensitive cell death [J].
Grant, M ;
Brown, I ;
Adams, S ;
Knight, M ;
Ainslie, A ;
Mansfield, J .
PLANT JOURNAL, 2000, 23 (04) :441-450
[23]   Nitric oxide improves internal iron availability in plants [J].
Graziano, M ;
Beligni, MV ;
Lamattina, L .
PLANT PHYSIOLOGY, 2002, 130 (04) :1852-1859
[24]   Identification of a plant nitric oxide synthase gene involved in hormonal signaling [J].
Guo, FQ ;
Okamoto, M ;
Crawford, NM .
SCIENCE, 2003, 302 (5642) :100-103
[25]   Nitric oxide represses the Arabidopsis floral transition [J].
He, YK ;
Tang, RH ;
Hao, Y ;
Stevens, RD ;
Cook, CW ;
Am, SM ;
Jing, LF ;
Yang, ZG ;
Chen, LG ;
Guo, FQ ;
Fiorani, F ;
Jackson, RB ;
Crawford, NM ;
Pei, ZM .
SCIENCE, 2004, 305 (5692) :1968-1971
[26]   The generation of Ca2+ signals in plants [J].
Hetherington, AM ;
Brownlee, C .
ANNUAL REVIEW OF PLANT BIOLOGY, 2004, 55 :401-427
[27]   Functional interaction of calmodulin with a plant cyclic nucleotide gated cation channel [J].
Hua, BG ;
Mercier, RW ;
Zielinski, RE ;
Berkowitz, GA .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2003, 41 (11-12) :945-954
[28]   Plants do it differently. A new basis for potassium/sodium selectivity in the pore of an ion channel [J].
Hua, BG ;
Mercier, RW ;
Leng, Q ;
Berkowitz, GA .
PLANT PHYSIOLOGY, 2003, 132 (03) :1353-1361
[29]   Identification of a pathogenicity island, which contains genes for virulence and avirulence, on a large native plasmid in the bean pathogen Pseudomonas syringae pathovar phaseolicola [J].
Jackson, RW ;
Athanassopoulos, E ;
Tsiamis, G ;
Mansfield, JW ;
Sesma, A ;
Arnold, DL ;
Gibbon, MJ ;
Murillo, J ;
Taylor, JD ;
Vivian, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (19) :10875-10880
[30]   Nitric oxide in plants: the biosynthesis and cell signalling properties of a fascinating molecule [J].
Lamotte, O ;
Courtois, C ;
Barnavon, L ;
Pugin, A ;
Wendehenne, D .
PLANTA, 2005, 221 (01) :1-4