Recombinant modified vaccinia virus Ankara efficiently restimulates human cytotoxic T lymphocytes in vitro

被引：9

作者：

Dorrell, L

O'Callaghan, CA

Britton, W

Hambleton, S

McMichael, A

Smith, GL

Rowland-Jones, S

Blanchard, TJ

机构：

[1] John Radcliffe Hosp, Inst Mol Med, Mol Immunol Grp, Oxford OX3 9DU, England

[2] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

[3] Centenary Inst, Newtown, NSW 2042, Australia

来源：

VACCINE | 2000年 / 19卷 / 2-3期

关键词：

cytotoxic T lymphocyte; vaccinia virus; MVA strain;

D O I：

10.1016/S0264-410X(00)00135-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immunogenicity of recombinant modified vaccinia Ankara, a highly attenuated vaccinia virus, expressing influenza nucleoprotein (MVA-NP) and HIV-1 gag (MVA-gag) was investigated. Restimulation of peripheral blood lymphocytes of healthy subjects with MVA-NP led to expansion of CTL with specificity for known NP epitopes. These CTL efficiently lysed NP peptide-pulsed targets and released interferon-gamma (IFN-gamma) on contact with epitope peptide. MVA-NP-stimulated CTL specific for the HLA-B8 epitope, NP380-88, stained with a tetrameric complex of HLA-B8 refolded with the NP380-88 peptide and anti-CD8 antibody on flow cytometry, CTL were also elicited from two HIV-I seropositive donors by restimulation with MVA-HIV-1 gag and showed specificity for immunodominant gag epitopes. These data indicate that restimulation of human CTL with recombinant MVA is effective and suggest that MVA will elicit CTL responses in humans in vivo. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：327 / 336

页数：10

共 31 条

[1] Recombinant vaccinia viruses for the characterization of Plasmodium falciparum-specific cytotoxic T lymphocytes: Recognition of processed antigen despite limited re-stimulation efficacy [J].