New generation of sterically protected C18 stationary phases containing embedded urea groups for use in high-performance liquid chromatography

被引：55

作者：

Silva, CR ^{[1
]}

Jardim, ICSF ^{[1
]}

Airoldi, C ^{[1
]}

机构：

[1] Univ Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 987卷 / 1-2期

关键词：

stationary phases; LC; urea stationary phases;

D O I：

10.1016/S0021-9673(02)01807-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

New monofunctional C-18 urea stationary phases with sterically protecting dimethyl and diisopropyl groups were prepared by a single step modification process. ProntoSil spherical silica (3 mum) was chemically modified with the monofunctional ethoxysilanes, [(3-octadecylurea)propylldimethyl and [(3-octadecylurea)propylldiisopropyl ethoxysilanes. The phases were characterized by elemental analysis, infrared and solid-state Si-29 and C-13 NMR spectroscopies. Chromatographic characterizations of the new phases in 50X3.9 mm HPLC columns were performed by the separation of two different test mixtures, containing nonpolar, polar and highly basic compounds. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：127 / 138

页数：12

共 28 条

[1] Improvement of gas chromatographic performance of cyelic siloxane bonded phases by incorporation of n-alkyl qroups [J].

Akapo, SO ;

Dimandja, JMD ;

Matyska, MT ;

Pesek, JJ .

ANALYTICAL CHEMISTRY, 1996, 68 (11) :1954-1959

[2] CHARACTERIZATION OF BONDED PHASES BY SOLID-STATE NMR-SPECTROSCOPY [J].

ALBERT, K ;

BAYER, E .

JOURNAL OF CHROMATOGRAPHY, 1991, 544 (1-2) :345-370

[3] Characterization and high performance liquid chromatographic evaluation of a new amide-functionalized reversed phase column [J].

Ascah, TL ;

Kallury, KMR ;

Szafranski, CA ;

Corman, SD ;

Liu, F .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1996, 19 (17-18) :3049-3073

[4] NOVEL, HIGHLY DEACTIVATED REVERSED-PHASE FOR BASIC COMPOUNDS [J].

ASCAH, TL ;

FEIBUSH, B .

JOURNAL OF CHROMATOGRAPHY, 1990, 506 :357-369

[5] Synthesis and solid-state NMR investigations of radiation-immobilized polysiloxanes on bare, titanium-grafted, and zirconium-grafted silicas [J].

Bachmann, S ;

Melo, LFC ;

Silva, RB ;

Anazawa, TA ;

Jardim, ICSF ;

Collins, KE ;

Collins, CH ;

Albert, K .

CHEMISTRY OF MATERIALS, 2001, 13 (05) :1874-1879

[6] PREPARATION OF VARIOUS BONDED PHASES FOR HPLC USING MONOCHLOROSILANES [J].

BERENDSEN, GE ;

PIKAART, KA ;

GALAN, LD .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1980, 3 (10) :1437-1464

[7] CHEMICALLY BONDED PHASES FOR THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF BASIC SUBSTANCES [J].

BUSZEWSKI, B ;

SCHMID, J ;

ALBERT, K ;

BAYER, E .

JOURNAL OF CHROMATOGRAPHY, 1991, 552 (1-2) :415-427

[8] CHROMATOGRAPHIC AND RELATED STUDIES OF ALKYLAMIDE PHASES [J].

BUSZEWSKI, B ;

KASTURI, P ;

GILPIN, RK ;

GANGODA, ME ;

JARONIEC, M .

CHROMATOGRAPHIA, 1994, 39 (3-4) :155-161

[9] INFLUENCE OF ELUENT COMPOSITION ON RETENTION AND SELECTIVITY OF ALKYLAMIDE PHASES UNDER REVERSED-PHASE CONDITIONS [J].

BUSZEWSKI, B ;

JARONIEC, M ;

GILPIN, RK .

JOURNAL OF CHROMATOGRAPHY A, 1994, 668 (02) :293-299

[10]

Collins KE, 2000, LC GC N AM, V18, P106

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