Age-related effects of TGF-β on proteoglycan synthesis in equine articular cartilage

被引：40

作者：

Iqbal, J ^{[1
]}

Dudhia, J ^{[1
]}

Bird, JLE ^{[1
]}

Bayliss, MT ^{[1
]}

机构：

[1] Univ London Royal Vet Coll, Dept Vet Basic Sci & Farm Anim & Equine Med & Sur, London NW1 0TU, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 274卷 / 02期

关键词：

cartilage; TGF-beta; proteoglycan; mRNA;

D O I：

10.1006/bbrc.2000.3167

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of proteoglycans was measured in normal equine articular cartilage of ages 9 months to 20 years and the effect of TGF-beta 1 on this activity was investigated. The rate of incorporation of [S-35]Na2SO4 decreased with age as did the responsiveness of the tissue to the growth factor. The enhanced synthesis of proteoglycan induced at all ages by TGF-beta 1 was down-regulated by IL-1 beta and retinoic acid. The expression of mRNA for TGF-beta 1, 2, and 3 was also measured, and although the level of TGF-beta 1 was highest at all ages, the expression of each growth factor decreased with age. (C) 2000 Academic Press.

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收藏

页码：467 / 471

页数：5

相关论文

共 29 条

[1] EXTRACTION AND ISOLATION OF MESSENGER-RNA FROM ADULT ARTICULAR-CARTILAGE [J].

ADAMS, ME ;

HUANG, DQ ;

YAO, LY ;

SANDELL, LJ .

ANALYTICAL BIOCHEMISTRY, 1992, 202 (01) :89-95

[2] TRANSFORMING GROWTH FACTOR-BETA CAUSES PARTIAL INHIBITION OF INTERLEUKIN-1 - STIMULATED CARTILAGE DEGRADATION INVITRO [J].

ANDREWS, HJ ;

EDWARDS, TA ;

CAWSTON, TE ;

HAZLEMAN, BL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 162 (01) :144-150

[3] Quantification of aggrecan and link-protein mRNA in human articular cartilage of different ages by competitive reverse transcriptase-PCR [J].

Bolton, MC ;

Dudhia, J ;

Bayliss, MT .

BIOCHEMICAL JOURNAL, 1996, 319 :489-498

[4] TRANSFORMING GROWTH FACTOR-BETA IS A POTENT INHIBITOR OF IL-1 INDUCED PROTEASE ACTIVITY AND CARTILAGE PROTEOGLYCAN DEGRADATION [J].

CHANDRASEKHAR, S ;

HARVEY, AK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (03) :1352-1359

[5]

Frazer A, 1994, Osteoarthritis Cartilage, V2, P235

[6]

Glansbeek HL, 1998, LAB INVEST, V78, P133

[7] GROWTH-FACTOR RESPONSIVENESS OF HUMAN ARTICULAR CHONDROCYTES - DISTINCT PROFILES IN PRIMARY CHONDROCYTES, SUBCULTURED CHONDROCYTES, AND FIBROBLASTS [J].

GUERNE, PA ;

SUBLET, A ;

LOTZ, M .

JOURNAL OF CELLULAR PHYSIOLOGY, 1994, 158 (03) :476-484

[8] GROWTH-FACTOR RESPONSIVENESS OF HUMAN ARTICULAR CHONDROCYTES IN AGING AND DEVELOPMENT [J].

GUERNE, PA ;

BLANCO, F ;

KAELIN, A ;

DESGEORGES, A ;

LOTZ, M .

ARTHRITIS AND RHEUMATISM, 1995, 38 (07) :960-968

[9] TRANSFORMING GROWTH-FACTOR-BETA-1 REGULATES TISSUE INHIBITOR OF METALLOPROTEINASES-1 EXPRESSION IN DIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES [J].

GUNTHER, M ;

HAUBECK, HD ;

VANDELEUR, E ;

BLASER, J ;

BENDER, S ;

GUTGEMANN, I ;

FISCHER, DC ;

TSCHESCHE, H ;

GREILING, H ;

HEINRICH, PC ;

GRAEVE, L .

ARTHRITIS AND RHEUMATISM, 1994, 37 (03) :395-405

[10] FLUOROMETRIC ASSAY OF DNA IN CARTILAGE EXPLANTS USING HOECHST-33258 [J].

KIM, YJ ;

SAH, RLY ;

DOONG, JYH ;

GRODZINSKY, AJ .

ANALYTICAL BIOCHEMISTRY, 1988, 174 (01) :168-176