Investigation of chromatographic performances and binding characteristics of BSA-encapsulated capillary column prepared by the sol-gel method

被引：49

作者：

Kato, M ^{[1
]}

Matsumoto, N ^{[1
]}

Sakai-Kato, K ^{[1
]}

Toyo'oka, T ^{[1
]}

机构：

[1] Univ Shizuoka, Sch Pharmaceut Sci, Dept Analyt Chem, Shizuoka 4228526, Japan

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2003年 / 30卷 / 06期

关键词：

sol-gel; capillary electrochromatography; protein-encapsulation; monolithic capillary column; enantioseparation;

D O I：

10.1016/S0731-7085(02)00527-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have developed a novel protein-encapsulation technique using the sol-gel method for the preparation of monolithic capillary columns for capillary electrochromatography. Bovine serum albumin (BSA) was encapsulated in tetramethoxysilane-based hydrogel and the enantio selectivity was evaluated. The present work examined the effect of various factors such as running buffer pH and concentration or organic modifier on the chromatographic performances as well as binding characteristics of BSA for D,L-tryptophan (Trp). The retention and enantioseparation dramatically changed depending on the buffer pH and concentrations. These factors influenced EOF and especially binding characteristics of BSA for L-Trp. Under the optimized conditions, the BSA-encapsulated columns revealed the adequate repeatability concerning column-to-column and run-to-run. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：1845 / 1850

页数：6

共 28 条

[1] DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .3. OPTICAL RESOLUTION OF A SERIES OF N-AROYL D,L-AMINO ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN COVALENTLY BOUND TO SILICA [J].

ALLENMARK, S ;

BOMGREN, B ;

BOREN, H .

JOURNAL OF CHROMATOGRAPHY, 1983, 264 (01) :63-68

[2] PROTEIN-BASED CAPILLARY AFFINITY GEL-ELECTROPHORESIS FOR THE SEPARATION OF OPTICAL ISOMERS [J].

BIRNBAUM, S ;

NILSSON, S .

ANALYTICAL CHEMISTRY, 1992, 64 (22) :2872-2874

[3]

BRINKER CJ, 1990, SOL GEL SCI PHYSICS

[4] CHIRAL SEPARATIONS BY COMPLEXATION WITH PROTEINS IN CAPILLARY ZONE ELECTROPHORESIS [J].

BUSCH, S ;

KRAAK, JC ;

POPPE, H .

JOURNAL OF CHROMATOGRAPHY, 1993, 635 (01) :119-126

[5] Characterisation of electroosmotic flow in capillary electrochromatography columns [J].

Dearie, HS ;

Spikmans, V ;

Smith, NW ;

Moffatt, F ;

Wren, SAC ;

Evans, KP .

JOURNAL OF CHROMATOGRAPHY A, 2001, 929 (1-2) :123-131

[6] SYNTHESIS AND CHROMATOGRAPHIC PROPERTIES OF AN HPLC CHIRAL STATIONARY PHASE BASED UPON HUMAN SERUM-ALBUMIN [J].

DOMENICI, E ;

BERTUCCI, C ;

SALVADORI, P ;

FELIX, G ;

CAHAGNE, I ;

MOTELLIER, S ;

WAINER, IW .

CHROMATOGRAPHIA, 1990, 29 (3-4) :170-176

[7] Photopolymerized sol-gel monoliths for capillary electrochromatography [J].

Dulay, MT ;

Quirino, JP ;

Bennett, BD ;

Kato, M ;

Zare, RN .

ANALYTICAL CHEMISTRY, 2001, 73 (16) :3921-3926

[8] ENCAPSULATION OF PROTEINS IN TRANSPARENT POROUS SILICATE-GLASSES PREPARED BY THE SOL-GEL METHOD [J].

ELLERBY, LM ;

NISHIDA, CR ;

NISHIDA, F ;

YAMANAKA, SA ;

DUNN, B ;

VALENTINE, JS ;

ZINK, JI .

SCIENCE, 1992, 255 (5048) :1113-1115

[9]

FULLERNOEL JK, 1972, J BIOL CHEM, V247, P7391

[10] LIQUID-CHROMATOGRAPHIC STUDIES OF THE EFFECT OF TEMPERATURE ON THE CHIRAL RECOGNITION OF TRYPTOPHAN BY SILICA-IMMOBILIZED BOVINE ALBUMIN [J].

GILPIN, RK ;

EHTESHAM, SE ;

GREGORY, RB .

ANALYTICAL CHEMISTRY, 1991, 63 (24) :2825-2828

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