A vector with the downstream box of the initiation codon can highly enhance protein expression in Escherichia coli

被引：14

作者：

Zhang, XL ^{[1
]}

Guo, P ^{[1
]}

Jing, GZ ^{[1
]}

机构：

[1] Acad Sinica, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 10期

关键词：

downstream box; expression vector; initiation codon;

D O I：

10.1023/A:1023563600459

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An expression vector, pET-DB, with a perfectly matching downstream box of the initiation codon has been constructed on the basis of the pET system. Any gene of interest can then be inserted into the vector. Four genes were used to test the expression efficiency of the vector. The results show that the vector pET-DB can further increase protein expression level at least up to 35-70% as compared with the initial T7 expression system, indicating that the downstream box can enhance protein expression in Escherichia coli.

引用

页码：755 / 760

页数：6

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