S-Nitrosoglutathione induces formation of nitrosylmyoglobin in isolated hearts during cardioplegic ischemia - An electron spin resonance study

被引：23

作者：

Konorev, EA ^{[1
]}

Joseph, J ^{[1
]}

Kalyanaraman, B ^{[1
]}

机构：

[1] MED COLL WISCONSIN,BIOPHYS RES INST,MILWAUKEE,WI 53226

来源：

FEBS LETTERS | 1996年 / 378卷 / 02期

关键词：

myocardium; S-nitrosothiol; nitrosylmyoglobin; ESR; ischemia-reperfusion injury;

D O I：

10.1016/0014-5793(95)01429-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, it has been shown that (NO)-N-. donor S-nitro-soglutathione (GSNO) improves the postischemic functional recovery in crystalloid buffer-perfused isolated rat hearts subjected to cardioplegic ischemia, Supplementation of cardioplegic solution with nitronyl nitroxide, a scavenger of (NO)-N-., antagonized this protective effect, Using low temperature ESR, we have detected nitrosylmyoglobin (MbNO) in rat hearts subjected to cardioplegic ischemia in the presence of GSNO (20- 200 mu mol/l). During aerobic reperfusion MbNO signal intensity gradually decreased, but persisted for up to 30 min of aerobic reperfusion, We conclude that MbNO is an endogenous marker of (NO)-N-. release in myocardial tissues, Implications of MbNO formation are discussed with respect to cardioprotection during ischemia- and reperusion-induced myocardial injury.

引用

页码：111 / 114

页数：4

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