Two-dimensional liquid chromatography study of the human whole saliva proteome

被引：116

作者：

Wilmarth, PA

Riviere, MA

Rustvold, DL

Lauten, JD

Madden, TE

David, LL

机构：

[1] Oregon Hlth Sci Univ, Sch Dent, Dept Integrat Biosci, Portland, OR 97239 USA

[2] Oregon Hlth Sci Univ, Sch Dent, Dept Periodontol, Portland, OR 97239 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2004年 / 3卷 / 05期

关键词：

human saliva; two-dimensional liquid chromatography; mass spectrometry;

D O I：

10.1021/pr049911o

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The human whole saliva proteome was investigated using two-dimensional liquid chromatography (2-DLC). The 2-DLC study was able to identify, with high confidence, 102 proteins including most known salivary proteins (35), and a large number of common serum proteins (67). Peptides from proline-rich proteins, abundant in saliva, had unusual cleavage sites and were frequently only partially tryptic. Three proteins not previously observed in human saliva were also detected. Significantly greater numbers of identified proteins, including high molecular weight, low molecular weight, and proline-rich proteins, were found with 2-DLC compared to previously reported two-dimensional gel electrophoresis studies.

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页码：1017 / 1023

页数：7

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