Presynaptic clustering of mGluR7a requires the PICK1PDZ domain binding site

被引：130

作者：

Boudin, H

Doan, A

Xia, J

Shigemoto, R

Huganir, RL

Worley, P

Craig, AM ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 USA

[2] Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA

[3] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

[4] Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Baltimore, MD 21205 USA

[5] CREST Japan Sci & Technol Corp, Natl Inst Physiol Sci, Okazaki, Aichi 4448585, Japan

来源：

NEURON | 2000年 / 28卷 / 02期

关键词：

D O I：

10.1016/S0896-6273(00)00127-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Aggregation of neurotransmitter receptors at pre- and postsynaptic structures is crucial for efficient neuronal communication. In contrast to the wealth of information about postsynaptic specializations, little is known about the molecular organization of presynaptic membrane proteins. We show here that the metabotropic glutamate receptor mGluR7a, which localizes specifically to presynaptic active zones, interacts in vitro and in vivo with PICK1. Coexpression in heterologous systems induces coclustering dependent upon the extreme C terminus of mGluR7a and the PDZ domain of PICK1, mGluR7a and PICK1 localize to excitatory synapses in hippocampal neurons. Furthermore, whereas transfected mGluR7a clusters at presynaptic sites, mGluR7a Delta3 lacking the PICK1 binding site targets to axons but does not cluster. These results suggest that PICK1 is a component of the presynaptic machinery involved in mGluR7a aggregation and in modulation of glutamate neurotransmission.

引用

页码：485 / 497

页数：13