Effective magnetic labeling of transplanted cells with HVJ-E for magnetic resonance imaging

被引：24

作者：

Toyoda, KI

Tooyama, I

Kato, M

Sato, H

Morikawa, S

Hisa, Y

Inubushi, T

机构：

[1] Shiga Univ Med Sci, Mol Neurosci Res Ctr, Otsu, Shiga 5202192, Japan

[2] Kyoto Prefectural Univ Med, Dept Otolaryngol, Kamigyo Ku, Kyoto 6028566, Japan

[3] Ishihara Sangyo Kaisya Ltd, Cent Res Inst, Kusatsu 5250025, Japan

来源：

NEUROREPORT | 2004年 / 15卷 / 04期

关键词：

Feridex; HVJ envelope; magnetic resonance imaging; Parkinson's disease; transfection; transplantation;

D O I：

10.1097/00001756-200403220-00004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Magnetic labeling of transplanted cells permits us to monitor their localization non-invasively using MRI. Since most transfection agents for magnetic labeling have the same cationic charge as Fe3+, the efficiency may be reduced. The hemagglutinating virus-envelope has no charge and utilizes membrane fusion activity to deliver internalized materials. In this study, we investigated the feasibility of using the envelope to incorporate paramagnetic Fe3+ particles into PC12 cells and astrocytes. The envelope effectively labeled both cells with Fe, which showed significant decreases of signal intensity in T2-weighted MRI. Labeled cells transplanted into the rat striatum were clearly visualized by T2*-weighted MRI at a magnetic field of 2T. The results indicate that the hemagglutinating virus-envelope is a powerful tool for magnetic labeling.

引用

收藏

页码：589 / 593

页数：5

相关论文

共 19 条

[1] TRANSPLANTATION OF ADRENAL-MEDULLARY TISSUE TO STRIATUM IN PARKINSONISM - 1ST CLINICAL-TRIALS [J].

BACKLUND, EO ;

GRANBERG, PO ;

HAMBERGER, B ;

KNUTSSON, E ;

MARTENSSON, A ;

SEDVALL, G ;

SEIGER, A ;

OLSON, L .

JOURNAL OF NEUROSURGERY, 1985, 62 (02) :169-173

[2] Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model [J].

Björklund, LM ;

Sánchez-Pernaute, R ;

Chung, SM ;

Andersson, T ;

Chen, IYC ;

McNaught, KS ;

Brownell, AL ;

Jenkins, BG ;

Wahlestedt, C ;

Kim, KS ;

Isacson, O .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (04) :2344-2349

[3] Monitoring stem cell therapy in vivo using magnetodendrimers as a new class of cellular MR contrast agents [J].

Bulte, JWM ;

Douglas, T ;

Witwer, B ;

Zhang, SC ;

Lewis, BK ;

van Gelderen, P ;

Zywicke, H ;

Duncan, ID ;

Frank, JA .

ACADEMIC RADIOLOGY, 2002, 9 :S332-S335

[4] THE USE OF GENETICALLY ALTERED ASTROCYTES TO PROVIDE NERVE GROWTH-FACTOR TO ADRENAL CHROMAFFIN CELLS GRAFTED INTO THE STRIATUM [J].

CUNNINGHAM, LA ;

HANSEN, JT ;

SHORT, MP ;

BOHN, MC .

BRAIN RESEARCH, 1991, 561 (02) :192-202

[5]

DUNNETT SB, 2000, NEURAL TRANSPLANTATI

[6] Fusigenic viral liposome for gene therapy in cardiovascular diseases [J].

Dzau, VJ ;

Mann, MJ ;

Morishita, R ;

Kaneda, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (21) :11421-11425

[7] The use of a recombinant lentiviral vector for ex vivo gene transfer into the rat CNS [J].

Englund, U ;

Ericson, C ;

Rosenblad, C ;

Mandel, RJ ;

Trono, D ;

Wictorin, K ;

Lundberg, C .

NEUROREPORT, 2000, 11 (18) :3973-3977

[8] Ex vivo and in vitro studies of transgene expression in rat astrocytes transduced with lentiviral vectors [J].

Ericson, C ;

Wictorin, K ;

Lundberg, C .

EXPERIMENTAL NEUROLOGY, 2002, 173 (01) :22-30

[9] Cellular and functional recovery of Parkinsonian rats after intrastriatal transplantation of carotid body cell aggregates [J].

Espejo, EF ;

Montoro, RJ ;

Armengol, JA ;

López-Barneo, J .

NEURON, 1998, 20 (02) :197-206

[10] Magnetic intracellular labeling of mammalian cells by combining (FDA-approved) superparamagnetic iron oxide MR contrast agents and commonly used transfection agents [J].

Frank, JA ;

Zywicke, H ;

Jordan, EK ;

Mitchell, J ;

Lewis, BK ;

Miller, B ;

Bryant, LH ;

Bulte, JWM .

ACADEMIC RADIOLOGY, 2002, 9 :S484-S487