Characterisation of a cyclin-dependent kinase (CDKA) gene expressed during somatic embryogenesis of coconut palm

被引：34

作者：

Montero-Cortes, Mayra ^{[1
]}

Rodriguez-Paredes, Francisco ^{[1
]}

Burgeff, Caroline ^{[1
]}

Perez-Nunez, Teresa ^{[1
]}

Cordova, Ivan ^{[1
]}

Oropeza, Carlos ^{[1
]}

Verdeil, Jean-Luc ^{[2
,3
]}

Saenz, Luis ^{[1
]}

机构：

[1] Ctr Invest Cient Yucatan, Biotechnol Unit, Merida 97200, Yucatan, Mexico

[2] Ctr Cooperat Int Rech Agron Dev, Montpellier 5, France

[3] Inst Rech Dev, Montpellier, France

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2010年 / 102卷 / 02期

关键词：

Cocos nucifera; Control of cell division; Somatic embryogenesis; CELL-CYCLE; NUCIFERA-L; LEAF EXPLANTS; ARABIDOPSIS; HOMOLOG; REGENERATION; COMPETENCE; TRANSITION; DIVISION; TISSUES;

D O I：

10.1007/s11240-010-9714-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The CDKA gene is linked to the cellular control. This gene was isolated from coconut palm (Cocos nucifera L) and a detailed expression analysis was done during somatic embryogenesis. Analysis of the deduced amino acid sequence showed the most important residues to be conserved. The highest homology was with Picea abies (96% similarity). Expression of the putative CnCDKA gene steadily increased during embryogenic callus formation phase when embryogenic competence is attained. In situ hybridization specified the localization of the transcripts as being mainly in a few cell layers within the meristematic centres in embryogenic calli at 90 day cultures. Analysis of CnCDKA expression at different somatic embryo formation stages showed that the expression was decreased progressively with the lowest expression in germinated somatic embryos.

引用

页码：251 / 258

页数：8

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