Helicobacter pylori in dental plaque and stomach of patients from Northern Brazil

被引：45

作者：

Assumpcao, Monica Barauna ^{[3
]}

Martins, Luisa Caricio ^{[4
]}

Melo Barbosa, Hivana Patricia ^{[1
]}

dos Santos Barile, Katarine Antonia ^{[1
]}

de Almeida, Sintia Silva ^{[1
]}

Assumpcao, Paulo Pimentel ^{[2
]}

de Oliveira Corvelo, Tereza Cristina ^{[1
]}

机构：

[1] Fed Univ Para, Immunogenet Lab, BR-66075110 Belem, Para, Brazil

[2] Joao de Barros Barreto Univ Hosp, Dept Surg, BR-66073000 Belem, Para, Brazil

[3] Joao de Barros Barreto Univ Hosp, Endoscopy Dept, BR-66073000 Belem, Para, Brazil

[4] Fed Univ Para, Nucleus Trop Med, Immunopathol Lab, BR-66075000 Belem, Para, Brazil

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2010年 / 16卷 / 24期

关键词：

Helicobacter pylori; Gastric mucosa; Dental plaque; cagA; vacA; ORAL COLONIZATION; INFECTION; TRANSMISSION; DNA; GASTRITIS; EPIDEMIOLOGY; PREVALENCE; VIRULENCE; DISEASE; ANTIGEN;

D O I：

10.3748/wjg.v16.i24.3033

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylon) retrieved from gastric mucosa and dental plaque in patients with dyspepsia. METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological examination, rapid urease test and polymerase chain reaction (PCR) assays to detect the presence of cagA and vacA polymorphisms. RESULTS: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR compared to histological examination and the rapid urease test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque samples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These virulent H. pylori isolates were involved in the severity of clinical outcome. CONCLUSION: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth. (C) 2010 Baishideng. All rights reserved.

引用

页码：3033 / 3039

页数：7

共 49 条

[1]

[Anonymous], 1989, Molecular Cloning: A Laboratory

[2] MOSAICISM IN VACUOLATING CYTOTOXIN ALLELES OF HELICOBACTER-PYLORI - ASSOCIATION OF SPECIFIC VACA TYPES WITH CYTOTOXIN PRODUCTION AND PEPTIC-ULCERATION [J].

ATHERTON, JC ;

CAO, P ;

PEEK, RM ;

TUMMURU, MKR ;

BLASER, MJ ;

COVER, TL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (30) :17771-17777

[3]

AYRES M, 2005, BIOESTAT 4 0 APLICAC, P125

[4] HELICOBACTER-PYLORI IN DENTAL PLAQUE [J].

BANATVALA, N ;

LOPEZ, CR ;

OWEN, R ;

ABDI, Y ;

DAVIES, G ;

HARDIE, J ;

FELDMAN, R .

LANCET, 1993, 341 (8841) :380-380

[5] Detection of Helicobacter pylori DNA in the oral cavity and gastroduodenal system of a Venezuelan population [J].

Berroteran, A ;

Perrone, M ;

Correnti, M ;

Cavazza, ME ;

Tombazzi, C ;

Goncalvez, R ;

Lecuna, V .

JOURNAL OF MEDICAL MICROBIOLOGY, 2002, 51 (09) :764-770

[6]

Cave D R, 1996, Am J Med, V100, p12S

[7]

CELLINI L, 1995, MICROBIOLOGICA, V18, P187

[8] HELICOBACTER-PYLORI IN MELBOURNE CHINESE IMMIGRANTS - EVIDENCE FOR ORAL-ORAL TRANSMISSION VIA CHOPSTICKS [J].

CHOW, TKF ;

LAMBERT, JR ;

WAHLQVIST, ML ;

HSUHAGE, BHH .

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 1995, 10 (05) :562-569

[9] Bacterial factors that mediate colonization of the stomach and virulence of Helicobacter pylori [J].

Clyne, Marguerite ;

Dolan, Brendan ;

Reeves, Emer P. .

FEMS MICROBIOLOGY LETTERS, 2007, 268 (02) :135-143

[10] Helicobacter pylori virulence and genetic geography [J].

Covacci, A ;

Telford, JL ;

Del Giudice, G ;

Parsonnet, J ;

Rappuoli, R .

SCIENCE, 1999, 284 (5418) :1328-1333

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