Class XI myosins are plant specific and responsible for cytoplasmic streaming. Because of the large number of myosin XI genes in angiosperms, it has been difficult to determine their precise role, particularly with respect to tip growth. The moss Physcomitrella patens provides an ideal system to study myosin XI function. P. patens has only two myosin XI genes, and these genes encode proteins that are 94% identical to each other. To determine their role in tip growth, we used RNA interference to specifically silence each myosin XI gene using 5' untranslated region sequences. We discovered that the two myosin XI genes are functionally redundant, since silencing of either gene does not affect growth or polarity. However, simultaneous silencing of both myosin XIs results in severely stunted plants composed of small rounded cells. Although similar to the phenotype resulting from silencing of other actin-associated proteins, we show that this phenotype is not due to altered actin dynamics. Consistent with a role in tip growth, we show that a functional, full-length fusion of monomeric enhanced green fluorescent protein (mEGFP) to myosin XI accumulates at a subcortical, apical region of actively growing protonemal cells.
机构:
Univ Massachusetts, Dept Biol, Amherst, MA 01003 USAUniv Massachusetts, Dept Biol, Amherst, MA 01003 USA
Augustine, Robert C.
;
Vidali, Luis
论文数: 0引用数: 0
h-index: 0
机构:
Univ Massachusetts, Dept Biol, Amherst, MA 01003 USAUniv Massachusetts, Dept Biol, Amherst, MA 01003 USA
Vidali, Luis
;
Kleinman, Ken P.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Dept Ambulatory Care & Prevent, Boston, MA 02215 USA
Harvard Pilgrim Hlth Care, Boston, MA 02215 USAUniv Massachusetts, Dept Biol, Amherst, MA 01003 USA
Kleinman, Ken P.
;
Bezanilla, Magdalena
论文数: 0引用数: 0
h-index: 0
机构:
Univ Massachusetts, Dept Biol, Amherst, MA 01003 USAUniv Massachusetts, Dept Biol, Amherst, MA 01003 USA
机构:
Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, JapanUniv Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Hashimoto, K
;
Igarashi, H
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Igarashi, H
;
Mano, S
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Mano, S
;
Nishimura, M
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Nishimura, M
;
Shimmen, T
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Shimmen, T
;
Yokota, E
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
机构:
Univ Massachusetts, Dept Biol, Amherst, MA 01003 USAUniv Massachusetts, Dept Biol, Amherst, MA 01003 USA
Augustine, Robert C.
;
Vidali, Luis
论文数: 0引用数: 0
h-index: 0
机构:
Univ Massachusetts, Dept Biol, Amherst, MA 01003 USAUniv Massachusetts, Dept Biol, Amherst, MA 01003 USA
Vidali, Luis
;
Kleinman, Ken P.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Dept Ambulatory Care & Prevent, Boston, MA 02215 USA
Harvard Pilgrim Hlth Care, Boston, MA 02215 USAUniv Massachusetts, Dept Biol, Amherst, MA 01003 USA
Kleinman, Ken P.
;
Bezanilla, Magdalena
论文数: 0引用数: 0
h-index: 0
机构:
Univ Massachusetts, Dept Biol, Amherst, MA 01003 USAUniv Massachusetts, Dept Biol, Amherst, MA 01003 USA
机构:
Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, JapanUniv Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Hashimoto, K
;
Igarashi, H
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Igarashi, H
;
Mano, S
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Mano, S
;
Nishimura, M
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Nishimura, M
;
Shimmen, T
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan
Shimmen, T
;
Yokota, E
论文数: 0引用数: 0
h-index: 0
机构:Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Harima, Hyogo 6781297, Japan