Myosin XI Is Essential for Tip Growth in Physcomitrella patens

被引:110
作者
Vidali, Luis [2 ]
Burkart, Graham M. [1 ]
Augustine, Robert C. [1 ]
Kerdavid, Erin [1 ]
Tuezel, Erkan [3 ]
Bezanilla, Magdalena [1 ]
机构
[1] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA
[2] Worcester Polytech Inst, Dept Biol & Biotechnol, Worcester, MA 01609 USA
[3] Worcester Polytech Inst, Dept Phys, Worcester, MA 01609 USA
基金
美国国家科学基金会;
关键词
ROOT HAIR; TAIL FUSIONS; LOCALIZATION; ELONGATION; CELLS; TOOL;
D O I
10.1105/tpc.109.073288
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Class XI myosins are plant specific and responsible for cytoplasmic streaming. Because of the large number of myosin XI genes in angiosperms, it has been difficult to determine their precise role, particularly with respect to tip growth. The moss Physcomitrella patens provides an ideal system to study myosin XI function. P. patens has only two myosin XI genes, and these genes encode proteins that are 94% identical to each other. To determine their role in tip growth, we used RNA interference to specifically silence each myosin XI gene using 5' untranslated region sequences. We discovered that the two myosin XI genes are functionally redundant, since silencing of either gene does not affect growth or polarity. However, simultaneous silencing of both myosin XIs results in severely stunted plants composed of small rounded cells. Although similar to the phenotype resulting from silencing of other actin-associated proteins, we show that this phenotype is not due to altered actin dynamics. Consistent with a role in tip growth, we show that a functional, full-length fusion of monomeric enhanced green fluorescent protein (mEGFP) to myosin XI accumulates at a subcortical, apical region of actively growing protonemal cells.
引用
收藏
页码:1868 / 1882
页数:15
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