Loss of WISP-2/CCN5 signaling in human pancreatic cancer: A potential mechanism for epithelial-mesenchymal-transition

被引：87

作者：

Dhar, Gopal

Mehta, Smita

Banerjee, Snlgdha

Gardner, Ashleigh

McCarty, Bryan M.

Mathur, Sharad C.

Campbell, Donald R.

Kambhampati, Suman

Banerjee, Sushanta K.

机构：

[1] Vet Adm Med Ctr, Canc Res Unit, Kansas City, MO 64128 USA

[2] Dept Hematol & Oncol, Kansas City, KS USA

[3] Dept Pathol, Kansas City, KS USA

[4] Univ Kansas, Med Ctr, Dept Anat & Cell Biol, Kansas City, KS 66103 USA

[5] Univ Missouri, St Lukes Hosp, Kansas City, KS USA

来源：

CANCER LETTERS | 2007年 / 254卷 / 01期

关键词：

pancreatic adenocarcinoma; WISP-2/CCN5; p53 tumor suppressor gene;

D O I：

10.1016/j.canlet.2007.02.012

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The objective of this study was to explore the pathophysiological relevance of WISP-2/CCN5 in progression of human pancreatic adenocarcinoma (PAC). We found WISP-2/CCN5 mRNA and protein expression was faint and sporadic in PAC and detected in only 8.7-20% of the samples with varying grades as compared to adjacent normal and chronic pancreatitis samples where expression was very high in the ducts and acini. Colocalization studies in tissue-microarray slides revealed WISP-2/CCN5 mRNA loss was associated with p53 overexpression in PAC. Like tissue samples, p53 mutant-PAC cell lines show loss of WISP-2/CCN5. Moreover, functional analysis studies demonstrate exposure of pancreatic cancer cells to WISP-2/CCN5 recombinant protein enhances mesenchymal-epithelial-transition (MET). Collectively, we suggest WISP-2/CCN5 silencing may be a critical event during differentiation and progression of PAC and mutant p53 is possibly an important player in pursuing this episode. Published by Elsevier Ltd.

引用

收藏

页码：63 / 70

页数：8

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