Purification and characterization of a beta-glucosidase from Polygonum tinctorium which catalyzes preferentially the hydrolysis of indican

被引：35

作者：

Minami, Y

Kanafuji, T

Miura, K

机构：

[1] Okayama University of Science, Faculty of Science, Department of Biochemistry, Okayama, 700

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 01期

关键词：

beta-glucosidase; glycoside; indican; indigo;

D O I：

10.1271/bbb.60.147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme, beta-glucosidase, which hydrolyzes indican (indoxyl-beta-D-glucoside) was isolated in pure form from a plant, Polygonum tinctorium, and characterized. The enzyme showed specifically a low K-m for indican (0.34 mM). The enzyme reacted with some other beta-glucosides having aromatic groups but not with alpha-glucoside or other glycosides like galactoside. The activity was inhibited by several metal cations such as Cu2+ and by typical inhibitors for beta-glucosidase such as D-glucono-1,5-lactone although these inhibitors required high concentrations.

引用

页码：147 / 149

页数：3

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